| Description |
1 online resource (xv, 412 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 440 |
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Methods in molecular biology (Clifton, N.J.) ; v. 440
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| Contents |
Vesicular trafficking : molecular tools and targets / Elena V. Vassilieva and Asma Nusrat -- Pharmacological inhibition of endocytic pathways : is it specific enough to be useful? / Andrei I. Ivanov -- In vitro assays to measure SNARE-mediated vesicle fusion / Susanne Kreye, Jörg Malsam, and Thomas H. Söllner -- Imaging Ca2+-triggered exocytosis of single secretory granules on plasma membrane lawns from neuroendocrine cells / Thorsten Lang -- FRAP analysis of secretory granule lipids and proteins in the sea urchin egg / Julian L. Wong and Gary M. Wessel -- A cell-free assay for endocytosis of E-cadherin / Toshiaki Sakisaka and Yoshimi Takai -- Cell-surface biotinylation to study endocytosis and recycling of occludin / Noriyuki Nishimura and Takuya Sasaki -- Fractionation of subcellular membrane vesicles of epithelial and nonepithelial cells by OptiPrep density gradient / Xuhang Li and Mark Donowitz -- Identification and characterization of interacting partners of Rab GTPases by yeast two-hybrid analyses / Mark Kail and Angelika Barnekow -- High-throughput analysis of the dynamics of recycling cell surface proteins / Roland Glovers, David E. James, and Adelle C.F. Coster -- Studying phagocytosis by live-cell scintillation proximity assay / Walter Stockinger and Axel Nohturfft -- Transcytosis of polymeric immunoglobin A in polarized Madin-Darby canine kidney cells / Asli Oztan, Christine Rondanino, and Gerard Apodaca -- The use of syntaxin chimeras to study polarized protein trafficking in epithelial cells / Martin B.A. ter Beest -- Targeting the epithelial SNARE machinery by bacterial neurotoxins / Véronique Proux-Gillardeaux and Thierry Galli -- Endocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor / Munekazu Yamakuchi [and others] -- Molecular dissection of HCL secretion in gastric parietal cells using streptolysin O permeabilization / Xia Ding . [and others] -- Addressing membrane protein topology using the fluorescence protease protection (FPP) assay / Holger Lorenz, Dale W. Hailey, and Jennifer Lippincott-Schwartz |
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Visualizing clathrin-mediated IgE receptor internalization by electron and atomic force microscopy / Alan R. Burns [and others] -- Analyzing endosomes in nonsectioned cells by transmission electron microscopy / Willem Stoorvogel -- Imaging exocytosis of single insulin secretory granules with TIRF microscopy / Shinya Nagamatsu and Mica Ohara-Imaizumi -- Reconstitution of depolarization and Ca2+-evoked secretion in Xenopus oocytes monitored by membrane capacitance / Roy Cohen, Bernhard M. Schmitt, and Daphne Atlas -- Low-noise recording of single-vesicle capacitance steps in cell-attached patches / Vitaly Klyachko, Zhenjie Zhang, and Meyer Jackson -- Good practices in single-cell amperometry / David J. Machado, Mónica S. Montesinos, and Ricardo Borges -- Analysis of single-vesicle exocytotic events recorded by amperometry / Eugene V. Mosharov -- Application of RNAi technology and fluorescent protein markers to study membrane traffic in Caenorhabditis elegans / Dmitry Poteryaev and Anne Spang -- FM 1-43 labeling of synaptic vesicle pools at the Drosophila neuromuscular junction / Patrik Verstreken, Tomoko Ohyama, and Hugo J. Bellen -- Probing E-cadherin endocytosis by morpholino-mediated Rab5 knockdown in zebrafish / Florian Ulrich and Carl-Philipp Heisenberg -- Quantifying endocytosis in vivo using intravital two-photon microscopy / Ruben M. Sandoval and Bruce A. Molitoris |
| Summary |
Due to their vital involvement in a wide variety of housekeeping and specialized cellular functions, exocytosis and endocytosis remain among the most popular subjects in biology and biomedical sciences. Tremendous progress in understanding these complex intracellular processes has been achieved by employing a wide array of research tools ranging from classical biochemical methods to modern imaging techniques. In Exocytosis and Endocytosis, skilled experts provide the most up-to-date, step-by-step laboratory protocols for examining molecular machinery and biological functions of exocytosis and endocytosis in vitro and in vivo. Following the highly successful Methods in Molecular Biology series format, the chapters present an introduction outlining the principle behind each technique, a list of the necessary materials, an easy to follow, readily reproducible protocol, and a Notes section offering tips on troubleshooting and avoiding known pitfalls. Insightful to both newcomers and seasoned professionals, Exocytosis and Endocytosis offers a unique and highly practical guide to versatile laboratory tools developed to study various aspects of intracellular vesicle trafficking in simple model systems and living organisms |
| Analysis |
exocytose |
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exocytosis |
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endocytose |
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endocytosis |
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celbiologie |
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cellular biology |
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Cellular Biology |
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Celbiologie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Endocytosis -- Laboratory manuals
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Exocytosis -- Laboratory manuals
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Endocytosis.
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Exocytosis.
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Cytology -- Technique.
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Endocytosis
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Exocytosis
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Cytological Techniques
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Cytology -- Technique
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Endocytosis
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Exocytosis
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Ivanov, Andrei I
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| ISBN |
9781597451789 |
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1597451789 |
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