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Author James, Eddie A

Title Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  


Description online resource (streaming video file)(942 seconds)
Series Biology
Summary This procedure demonstrates the purification and in vitro expansion of antigen specific CD4+ T cells from whole peripheral blood and their visualization using MHC class II tetramers. Tetramers permit the direct visualization of T cells with a single antigen specificity and defined MHC class II restriction
Notes Title from resource description page
Audience For undergraduate, graduate, and professional students
Notes English
Subject Biology
Form Streaming video