| Description |
1 online resource (xii, 411 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1936 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1936. 1940-6029
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| Contents |
Single-cell RNA sequencing of oligodendrocyte lineage cells from the mouse central nervous system / Sueli Marques, David van Bruggen, and Goncalo Castelo-Branco -- Isolation and preparation of cells from focal remyelinating central nervous system lesions for RNA sequencing / Claire L. Davies, Stefka Gyoneva, Anne Cotleur, Richard M. Ransohoff, and Veronique E. Miron -- Myelin : methods for purification and proteome analysis / Michelle S. Erwig, Dorte Hesse, Ramona B. Jung, Marina Uecker, Kathrin Kusch, Stefan Tenzer, Olaf Jahn, and Hauke B. Werner -- In vitro generation and electrophysiological characterization / Dario Magnani, Siddharthan Chandran, David J.A. Wyllie, and Matthew R. Livesey -- Isolation and culture of oligodendrocytes / Marie-Theres Weil, Giselheid Schulz-Eberlin, Chaitali Mukherjee, Wen Ping Kuo-Elsner, Isabelle Schafer, Christina Muller, and Mikael Simons -- Neuron-free microfiber assay to assess myelin sheath formation / Marie E. Bechler -- Oligodendrocyte-neuron myelinating coculture / Matthew Swire and Charles ffrench-Constant -- Teasing of ventral spinal cord white matter fibers for the analysis of central nervous system nodes of ranvier / Andrew A. Jarjour and Diane L. Sherman-- Whole-cell patch clamp recordings from oligodendrocyte lineage cells in brain slices / Sylvia Agathou and Ragnhildur Thora Karadottir -- Ex vivo slice cultures to study myelination, demyelination, and remyelination in mouse brain and spinal cord / Sowmya Sekizar and Anna Williams -- Forward genetic screen using zebrafish to identify new genes involved in myelination / Linde Kegel, Maria Rubio, Rafael G. Almeida, Silvia Benito, Anna Klingseisen, and David A. Lyons -- Manipulating neuronal activity in the developing zebrafish spinal cord to investigate adaptive myelination / Jill M. Williamson, David A. Lyons, and Rafael G. Almeida -- Drug-inducible transgenic zebrafish model for myelinating glial cell ablation / Marja J. Karttunen and David A. Lyons -- Conditional demyelination and remyelination in a transgenic Xenopus laevis / Abdelkrim Mannioui and Bernard Zalc -- Conditional mutagenesis in oligodendrocyte lineage cells / Sandra Goebbels and Klaus-Armin Nave -- Recent advances in live imaging of cells of the oligodendrocyte lineage / Jaime Eugenin von Bernhardi and Leda Dimou -- DTA mouse model for oligodendrocyte ablation and CNS demyelination / Maria Traka -- Human glial chimeric mice to define the role of glial pathology in human disease / John N. Mariani, Lisa Zou, and Steven A. Goldman -- Method to detect cytochrome c oxidase activity and mitochondrial proteins in oligodendrocytes / Graham R. Campbell and Don J. Mahad -- Transmission electron microscopy of oligodendrocytes and myelin / Marie-Theres Weil, Torben Ruhwedel, Martin Meschkat, Boguslawa Sadowski, and Wiebke Mobius -- Toxin-based models to investigate demyelination and remyelination / Christopher E. McMurran, Chao Zhao, and Robin J.M. Franklin -- Magnetic resonance techniques for imaging white matter / Cassandra Sampaio-Baptista, Kata Diosi, and Heidi Johansen-Berg |
| Summary |
This volume looks at the study of oligodendrocytes through in vitro and in vivo techniques, multiple model organisms, using approaches that bridge scales from molecular through system. Chapters in this book cover topics such as fundamental molecular analyses of oligodendrocytes and myelin; in vitro, ex vivo, and in vivo molecular-cellular-electrophysiology-based techniques; oligodendrocyte formation, homeostasis, and disruption in zebrafish and Xenopus; and parallel system-level imaging of animal and human models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Oligodendrocytes: Methods and Protocols is a valuable reference guide that highlights the expansive and fast-paced nature of research into oligodendrocyte biology underlying health and function |
| Notes |
Print version record |
| Subject |
Oligodendroglia -- Laboratory manuals
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Oligodendroglia.
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Oligodendroglia
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Neurosciences.
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Cellular biology (cytology)
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Medical -- Neuroscience.
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Oligodendroglia
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Lyons, David A., editor
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Kegel, Linde, editor
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| ISBN |
9781493990726 |
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1493990721 |
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