| Description |
1 online resource (xxv, 415 pages) : illustrations |
| Contents |
The Sampling and Sample Preparation Problem in Microbial Metabolomics / Walter M van Gulik, André B Canelas, Reza M Seifar, Joseph J Heijnen -- Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics / Gérard Hopfgartner, Emmanuel Varesio -- Uncertainty of Measurement in Quantitative Metabolomics / Raffaele Guerrasio, Christina Haberhauer-Troyer, Stefan Neubauer, Kristaps Klavins, Madeleine Werneth, Gunda Koellensperger, Stephen Hann -- Gas Chromatography and Comprehensive Two-Dimensional Gas Chromatography Hyphenated with Mass Spectrometry for Targeted and Nontargeted Metabolomics / Song Yang, Jamin C Hoggard, Mary E Lidstrom, Robert E Synovec -- LC-MS-Based Nontargeted Metabolomics / Georgios A Theodoridis, Helen G Gika, Ian D Wilson -- The Potential of Ultrahigh Resolution MS (FTICR-MS) in Metabolomics / Franco Moritz, Sara Forcisi, Mourad Harir, Basem Kanawati, Marianna Lucio, Dimitrios Tziotis, Philippe Schmitt-Kopplin -- The Art and Practice of Lipidomics / Koen Sandra, Ruben t'kindt, Lucie Jorge, Pat Sandra -- The Role of CE-MS in Metabolomics / Rawi Ramautar, Govert W Somsen, Gerhardus J de Jong -- NMR-Based Metabolomics Analysis / Andrea Lubbe, Kashif Ali, Robert Verpoorte, Young Hae Choi -- Potential of Microfluidics and Single Cell Analysis in Metabolomics (Micrometabolomics) / Meghan M Mensack, Ryan E Holcomb, Charles S Henry -- Data Processing in Metabolomics / Age K Smilde, Margriet M W B Hendriks, Johan A Westerhuis, Huub C J Hoefsloot -- Metabolic Flux Analysis / Christoph Wittmann, Jean-Charles Portais -- Metabolomics: Application in Plant Sciences / Gaetan Glauser, Julien Boccard, Jean-Luc Wolfender, Serge Rudaz -- Metabolomics and Its Role in the Study of Mammalian Systems / Warwick B Dunn, Mamas Mamas, Alexander Heazell -- Metabolomics in Biotechnology (Microbial Metabolomics) / Marco Oldiges, Stephan Noack, Nicole Paczia -- Nutritional Metabolomics / Hannelore Daniel |
| Summary |
Unlike other handbooks in this emerging field, this guide focuses on the challenges and critical parameters in running a metabolomics study, including such often-neglected issues as sample preparation, choice of separation and detection method, recording and evaluating data as well as method validation. By systematically covering the entire workflow, from sample preparation to data processing, the insight and advice offered here helps to clear the hurdles in setting up and running a successful analysis, resulting in high-quality data from every experiment. Based on more than a decade of practical experience in developing, optimizing and validating metabolomics approaches as a routine technology in the academic and industrial research laboratory, the lessons taught here are highly relevant for all systems-level approaches, whether in systems biology, biotechnology, toxicology or pharmaceutical sciences. From the Contents: * Sampling and Sample Preparation in Microbial Metabolomics * Tandem Mass Spectrometry Hyphenated with HPLC and UHPLC for Targeted Metabolomics * GC-MS, LC-MS, CE-MS and Ultrahigh Resolution MS (FTICR-MS) in Metabolomics * NMR-based metabolomics analysis * Potential of Microfluidics and Single Cell Analysis in Metabolomics * Data Processing in Metabolomics * Validation and Measurement Uncertainty in Metabolomic Studies * Metabolomics and its Role in the Study of Mammalian Systems and in Plant Sciences * Metabolomics in Biotechnology and Nutritional Metabolomics and more |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Description based on print version record |
| Subject |
Metabolism.
|
|
Metabolism -- Research -- Methodology
|
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Metabolomics
|
|
Metabolism
|
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metabolism (biological concept)
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MEDICAL -- Nutrition.
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Metabolism
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Metabolism -- Research -- Methodology
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| Form |
Electronic book
|
| Author |
Lämmerhofer, Michael, editor
|
|
Weckwerth, Wolfram, editor
|
| LC no. |
2012554203 |
| ISBN |
9783527655861 |
|
3527655867 |
|
9783527655892 |
|
3527655891 |
|
9783527655885 |
|
3527655883 |
|
9783527655878 |
|
3527655875 |
|
9781299313545 |
|
129931354X |
|
