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Author Maggioli, Mayara F

Title Application of Long-term cultured Interferon-γ Enzyme-linked Immunospot Assay for Assessing Effector and Memory T Cell Responses in Cattle
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  


Description online resource (streaming video file)(957 seconds)
Series Immunology and Infection
Summary Long-term cultured interferon-γ enzyme-linked immunospot assay is used as a measure of central memory responses and correlates with protective anti-mycobacterial vaccine responses. With this assay, peripheral blood mononuclear cells are stimulated with mycobacterial antigens and interleukin-2 for 14 days, enabling differentiation and expansion of central memory T cells
Notes Title from resource description page
Audience For undergraduate, graduate, and professional students
Notes English
Subject Immunology and Infection
Form Streaming video