| Description |
1 online resource (xii, 196 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 373 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 373.
|
| Contents |
The history of pyrosequencing / Pål Nyrén -- Pyrosequencing applications / Sharon Marsh -- Web-based primer design software for genome-scale genotyping by pyrosequencing / Steven Ringquist [and others] -- Pyrosequencing : a simple method for accurate genotyping / Cristi R. King and Tiffany Scott-Horton -- Universal primer applications for pyrosequencing / Dong-Chuan Guo and Dianna M. Milewicz -- Allele quantification and DNA pooling methods / Jonathon Wasson -- Multiplex pyrosequencing for DNA variation analysis / Pritesh Patel, Yih-Horng Shiao, and Paolo Fortina -- Analysis of gene-specific DNA methylation patterns by pyrosequencing technology / Jörg Tost and Ivo Glynne Gut -- Detecting mutations that confer oxazolidinone resistance in gram-positive bacteria / Neil Woodford, Sarah E. North, and Matthew J. Ellington -- Pyrosequencing-based strategies for improved allele typing of human leukocyte antigen loci / Steven Ringquist [and others] -- Pyrosequencing of phage display libraries for the identification of cell-specific targeting ligands / Ahad A. Rahim -- Gene copy number detection in animal studies / Gerli Pielberg and Leif Andersson -- Detection of allelic imbalance in gene expression using pyrosequencing / Hua Wang and Steven C. Elbein -- Pyrosequencing genotype storage techniques / Derek J. Van Booven |
| Summary |
Pyrosequencing® Protocols presents detailed protocols for the multidisciplinary application of Pyrosequencing® technology, all written by world-renowned experts in the field. This comprehensive volume enables quick reference by collecting the primary applications for Pyrosequencing®, and supplementing each protocol with troubleshooting tips specific to that method. Chapters 1 and 2 present an introductory overview of the origins of the Pyrosequencing® methodology and its key applications. Chapters 3 and 4 describe primer selection and the basic Pyrosequencing® technique, and Chapters 5-7 provide methods for improving throughput and decreasing cost. Detailed applications for Pyrosequencing® comprise Chapters 8-13, and the important aspects of data storage is discussed in Chapter 14. This comprehensive volume both highlights the versatility of and provides detailed protocols for the application of Pyrosequencing® |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
DNA -- Analysis
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Nucleotide sequence.
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Methodology.
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Genetics -- Technique.
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Sequence Analysis, DNA -- methods
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Methods
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Sequence Analysis, DNA
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Investigative Techniques
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Sequence Analysis
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Genetic Techniques
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methodology.
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MEDICAL -- Genetics.
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Sequence Analysis, DNA -- methods.
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DNA -- Analysis.
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Nucleotide sequence.
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DNA -- Analysis
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Nucleotide sequence
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| Genre/Form |
Methods (Music)
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
|
| Author |
Marsh, Sharon
|
| ISBN |
1588296458 |
|
9781588296450 |
|
1597453773 |
|
9781597453776 |
|
