| Description |
1 online resource (xi, 326 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; 398 |
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Methods in molecular biology (Clifton, N.J.) ; 398.
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| Contents |
Overview of membrane rafts / Thomas J. McIntosh -- Analysis of raft affinity of membrane proteins by detergent-insolubility / Deborah A. Brown -- Nondetergent isolation of rafts / Mehul B. Shah and Pravin B. Sehgal -- Detecting ordered domain formation (lipid rafts) in model membranes using tempo / Omar Bakht and Erwin London -- Using monomolecular films to characterize lipid lateral interactions / Rhoderick E. Brown and Howard L. Brockman -- Electro-formation and fluorescence microscopy of giant vesicles with coexisting liquid phases / Sarah L. Veatch -- Fluorescence correlation spectroscopy / Kristen Bacia and Petra Schwille -- Multiphoton laser-scanning microscopy and spatial analysis of dehydroergosterol distributions on plasma membrane of living cells / Avery L. McIntosh [and others] -- NMR detection of lipid domains / Ivan V. Polozov and Klaus Gawrisch -- Lateral diffusion coefficients of raft lipids from pulsed field gradient NMR / Greger Orädd and Göran Lindblom -- Saturation-recovery electron paramagnetic resonance discrimination by oxygen transport (DOT) method for characterizing membrane domains / Witold K. Subczynski [and others] -- Plasmon-waveguide resonance spectroscopy studies of lateral segregation in solid-supported proteolipid bilayers / Zdzislaw Salamon, Savitha Devanathan, and Gordon Tollin -- Fluorescence recovery after photobleaching studies of lipid rafts / Anne K. Kenworthy -- Single-molecule tracking / Marija Vrljic, Stefanie Y. Nishimura, and W.E. Moerner -- X-ray diffraction to determine the thickness of raft and nonraft bilayers / Thomas J. McIntosh -- Small-angle neutron scattering to detect rafts and lipid domains / Jeremy Pencer [and others] -- Exploring membrane domains using native membrane sheets and transmission electron microscopy / Bridget S. Wilson [and others] -- Atomic force microscopy of lipid domains in supported model membranes / Alan R. Burns |
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Atomistic and coarse-grained computer simulations of raft-like lipid mixtures / Sagar A. Pandit and H. Larry Scott -- A microscpic model calculation of the phase diagram of ternary mixtures of cholesterol and saturated and unsaturated phospholipids / R. Elliott, I. Szleifer, and M. Schick |
| Summary |
There are many fundamental unanswered questions concerning the composition, structure, and dynamics of lateral microdomains (rafts) in biological membranes. Thus, sophisticated techniques have been used to study intact cell membranes as well as model systems composed of specific lipids and proteins thought to be in rafts. Using biochemical, biophysical, and computational methods, Lipid Rafts provides the most up-to-date information on the subject, information that promises to continue unraveling the many mysteries of the properties and formation of lipid rafts. Included in Lipid Rafts are insightful discussions concerning chemical composition of rafts, monolayer techniques, fluorescence correlation spectroscopy, nuclear magnetic resonance (NMR) techniques, electron paramagnetic resonance (EPR) applications, fluorescence recovery after photobleaching (FRAP), X-ray diffraction, and more. The many biochemical, biophysical, and computational methods described in this book have already provided detailed information on lipid rafts and promise to continue to unravel many more mysteries concerning raft formation and properties. All this makes Lipid Rafts an essential reference for molecular biologists, biochemists, biophysicists, and biophysical chemists |
| Analysis |
celmembranen |
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cell membranes |
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fysicochemische eigenschappen |
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physicochemical properties |
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biochemie |
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biochemistry |
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Biochemistry |
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Biochemie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Membrane lipids.
|
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Lipids.
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Membrane Microdomains
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Membrane Lipids
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Lipids
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lipid.
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SCIENCE -- Life Sciences -- Biochemistry.
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Membrane Lipids.
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Membrane Microdomains.
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Lipids.
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Lipids
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Membrane lipids
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| Genre/Form |
Laboratory manuals.
|
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
McIntosh, Thomas J
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| ISBN |
9781597455138 |
|
159745513X |
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1588297292 |
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9781588297297 |
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1280971908 |
|
9781280971907 |
|
9786610971909 |
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6610971900 |
|
