| Description |
1 online resource (xi, 245 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1328 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1328. 1064-3745
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| Contents |
Drosophila melanogaster oogenesis : an overview / John M. McLaughlin and Diana P. Bratu -- Basic techniques in Drosophila ovary preparation / Letitia Thompson, Kristen Randolph, and Amanda Norvell -- Mosaic analysis in the Drosophila melanogaster ovary / Thomas Rubin and Jean-René Huynh -- Genetic mosaic analysis of stem cell lineages in the Drosophila ovary / Kaitlin M. Laws and Daniela Drummond-Barbosa -- Culturing Drosophila egg chambers and investigating developmental processes through live imaging / Lathiena Manning and Michelle Starz-Gaiano -- Border cell migration : a model system for live imaging and genetic analysis of collective cell movement / Mohit Prasad [and others] -- Visualizing microtubule networks during Drosophila oogenesis using fixed and live Imaging / Kevin Legent, Nicolas Tissot, and Antoine Guichet -- Visualization of actin cytoskeletal dynamics in fixed and live Drosophila egg chambers / Christopher M. Groen and Tina L. Tootle -- Single-molecule RNA in situ hybridization (smFISH) and immunofluorescence (IF) in the Drosophila egg chamber / Livia V. Bayer [and others] -- Fluorescent in situ hybridization of nuclear bodies in Drosophila melanogaster ovaries / Zehra F. Nizami, Ji-Long Liu, and Joseph G. Gall -- Ultrastructural analysis of Drosophila ovaries by electron microscopy / Thomas R. Hurd [and others] -- Immuno-electron microscopy and electron microscopic in situ hybridization for visualizing piRNA biogenesis bodies in Drosophila ovaries / Shinsuke Shibata [and others] -- Visualizing cytoophidia expression in Drosophila follicle cells via immunohistochemistry / Ömür Y. Tastan and Ji-Long Liu -- Detection of cell death and phagocytosis in the Drosophila ovary / Tracy L. Meehan [and others] -- Analysis of cell cycle switches in Drosophila oogenesis / Dongyu Jia, Yi-Chun Huang, and Wu-Min Deng -- Global run-on sequencing (GRO-seq) library preparation from Drosophila ovaries / Nikolay V. Rozhkov -- Bioinformatics analysis to identify RNA-protein interactions in oogenesis / Ravinder Singh |
| Summary |
This volume provides current up-to-date protocols for preparing the ovary for various imaging techniques, genetic protocols for generating mutant clones, mosaic analysis and assessing cell death. Chapters address methods for performing genome wide gene expression analysis and bioinformatics for studies of RNA-protein interactions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Drosophila Oogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Drosophila -- Genetics -- Laboratory manuals
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Drosophila -- genetics
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Oogenesis -- genetics
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Animal pathology & diseases.
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Cellular biology (cytology)
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Science -- Life Sciences -- Zoology -- General.
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Drosophila -- Genetics
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Bratu, Diana P., editor.
|
|
McNeil, Gerard P., editor.
|
| ISBN |
9781493928514 |
|
1493928511 |
|
1493928503 |
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9781493928507 |
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