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Title Signal transduction protocols / edited by Robert C. Dickson and Michael D. Mendenhall
Edition Second edition
Published Totowa, N.J. : Humana Press, [2004]


Location Call no. Vol. Availability
 W'PONDS  574.876 Ken/Stp 2004  AVAILABLE
Description xii, 327 pages : illustrations ; 24 cm
Series Methods in molecular biology ; 284
Methods in molecular biology (Clifton, N.J.) ; v. 284
Contents Making protein immunoprecipitates / Elaine A. Elion and Yunmei Wang -- Signal transduction inhibitors in cellular function / Maofu Fu ... [et al.] -- Two-dimensional gel electrophoresis for the identification of signaling targets -- Yukihito Kabuyama ... [et al.] -- A high-throughput mammalian cell-based transient transfection assay / Daniel J. Noonan, Kenneth Henry, and Michelle L. Twaroski -- Determining protein half-lives / Pengbo Zhou -- Assaying protein kinase activity / Jan Brbek and Steven K. Hanks -- Comparative phosphorylation site mapping from gel-derived proteins using a multidimensional ES/MS-based approach / Francesca Zappacosta, Michael J. Huddleston and Roland S. Annan -- Studies of calmodulin-dependent regulation / Paul C. Brandt and Thomas C. Vanaman -- Measurement of protein/DNA interactions in vivo by chromatin immunoprecipitation -- Hogune Im ... [et al.] --
Characterization of protein-DNA association in vivo by chromatin immunoprecipitation / Laurent Kuras -- Nonradioactive methods for detecting activation of ras-related small G proteins / Douglas A. Andres -- Nucleocytoplasmic glycosylation, o-glcnac: identification and site mapping / Natasha Elizabeth Zachara, Win Den Cheung,and Gerald Warren Hart -- Techniques in protein methylation / Jaeho Lee, Donghang Cheng, and Mark T. Bedford -- Assaying lipid phosphate phosphatase activities / Gil-Soo Han and George M. Carman -- Assaying phosphoinositide phosphatases / Gregory S. Taylor and Jack E. Dixon -- Assaying phospholipase A2 activity / Christina C. Leslie and Michael H. Gelb -- Measurement and immunofluorescence of cellular phosphinositides / Hiroko Hama ... [et al.] -- Measuring dynamic changes in camp using fluorescence resonance energy transfer / Sandrine Evellin ... [et al.] -- In vivo detection of protein/protein interaction in plant cells using BRET / Chitra Subramanian ... [et al.] -- Revealing protein dynamics by photobleaching techniques / Frank van Drogen and Matthias Peter -- Assaying cytochrome C translocation during apoptosis / Nigel J. Waterhouse ... [et al.]
Bibliography Includes bibliographical references and index
Subject Cellular signal transduction -- Laboratory manuals.
G proteins -- Laboratory manuals.
Cell receptors -- Laboratory manuals.
GTP-Binding Proteins -- analysis.
Signal Transduction -- physiology.
Genre/Form Laboratory Manual.
Laboratory manuals.
Author Dickson, Robert C.
Mendenhall, Michael Dean.
LC no. 2004005164
ISBN 1588292452 alkaline paper
1592598161 E-ISBN