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Author Mijaljica, Dalibor

Title A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (627 secs.)
Series Biology
Summary A robust approach to monitor the delivery of organelles to the acidic lumen of the yeast vacuole for degradation and recycling is described. The method relies on the specific labeling of target organelles with a genetically encoded dual-emission fluorescence pH-biosensor, and visualization of individual cells using fluorescence microscopy
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Biology
Form Streaming audio