| Description |
1 online resource |
| Contents |
Cover ; Title page; Copyright page; Contents; List of Contributors; Preface; Chapter 1 -- Mass spectrometry in the clinical laboratory: determining the need and avoiding pitfalls; 1 -- Clinical mass spectrometry; 1.1 -- Basic mass spectrometry concepts; 1.2 -- Common ion sources for clinical mass spectrometry; 1.2.1 -- Electrospray Ionization (ESI); 1.2.2 -- Atmospheric Pressure Chemical Ionization (APCI); 1.2.3 -- Atmospheric Pressure Photoionization (APPI); 1.3 -- Commonly used mass analyzers; 1.3.1 -- Quadrupole Mass Analyzers; 1.3.2 -- Time-of-Flight Mass Analyzers; 1.3.3 -- Ion Trap Mass Analyzers |
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1.3.4 -- Hybrid Tandem Mass Analyzers1.4 -- Determining the need for clinical LC-MS; 1.5 -- Potential pitfalls and addressing the challenges; 1.5.1 -- External Quality Assessment and Quality Control; 1.5.2 -- Matrix Effects and Interferences; 1.5.3 -- Technical Hurdles; 1.5.4 -- Practical Considerations; References; Chapter 2 -- Application specific implementation of mass spectrometry platform in clinical laboratories; 1 -- Mass spectrometry versus automated immunoassay platforms; 2 -- Early decisions in the implementation of mass spectrometry in the laboratory |
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3 -- Importance of internal standards in mass spectrometry4 -- Matrix effects, extraction efficiency, and ion suppression/enhancement; 5 -- Analyte extraction; 6 -- Choice of separation technique: GC versus LC; 7 -- Choice of stationary phase; 8 -- Choice of ionization techniques; 9 -- Choice of mass analyzer; 10 -- Summary; References; Chapter 3 -- Sample preparation techniques for mass spectrometry in the clinical laboratory; 1 -- Why is sample preparation needed for LC-MS/MS?; 2 -- Selecting a sample preparation protocol; 3 -- Description of commonly used clinical sample preparation protocols |
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3.1 -- Simple-dilution sample preparation protocol (DIL)3.2 -- Simple-protein precipitation sample preparation protocol (PPT); 3.3 -- More complex-liquid-liquid extraction sample preparation protocol (LLE); 3.4 -- Commercial media-phospholipid removal sample preparation protocol (PLR); 3.5 -- Commercial media-supported liquid extraction sample preparation protocol (SLE or SALL); 3.6 -- Commercial media-solid phase extraction sample preparation protocol (SPE); 3.7 -- Online SPE; 4 -- Evaluation of sample preparation protocols; 4.1 -- Evaluating chromatography |
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4.2 -- Evaluating signal to noise (S/N) at the LLOQ4.3 -- Evaluating matrix effect (ME); 4.3.1 -- Quantitative Matrix Effect Experiment; 4.3.2 -- Qualitative Postcolumn Matrix Effect Experiment; 4.3.3 -- Phospholipid Direct Detection Experiment; 4.3.4 -- Matrix Mixing Experiment; 4.4 -- Evaluating method performance; 4.5 -- Evaluating practicality; 4.6 -- Evaluating robustness; 5 -- Comparison of sample preparation protocols; 6 -- Optimization of sample preparation protocols; 6.1 -- Generic-optimizing for 96-well format; 6.2 -- Generic-optimizing LC injection solvent composition |
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6.2.1 -- Analyte Solubility |
| Summary |
Mass Spectrometry for the Clinical Laboratory is an accessible guide to mass spectrometry and the development, validation, and implementation of the most common assays seen in clinical labs. It provides readers with practical examples for assay development, and experimental design for validation to meet CLIA requirements, appropriate interference testing, measuring, validation of ion suppression/matrix effects, and quality control. These tools offer guidance on what type of instrumentation is optimal for each assay, what options are available, and the pros and cons of each. Readers will find a full set of tools that are either directly related to the assay they want to adopt or for an analogous assay they could use as an example. Written by expert users of the most common assays found in a clinical laboratory (clinical chemists, toxicologists, and clinical pathologists practicing mass spectrometry), the book lays out how experts in the field have chosen their mass spectrometers, purchased, installed, validated, and brought them on line for routine testing. The early chapters of the book covers what the practitioners have learned from years of experience, the challenges they have faced, and their recommendations on how to build and validate assays to avoid problems. These chapters also include recommendations for maintaining continuity of quality in testing. The later parts of the book focuses on specific types of assays (therapeutic drugs, Vitamin D, hormones, etc.). Each chapter in this section has been written by an expert practitioner of an assay that is currently running in his or her clinical lab |
| Bibliography |
Includes bibliographical references and index |
| Subject |
Mass spectrometry.
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Diagnosis, Laboratory.
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Medical laboratory technology.
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Mass Spectrometry
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Clinical Laboratory Techniques
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mass spectrometry.
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SCIENCE -- Chemistry -- Analytic.
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Medical laboratory technology
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Diagnosis, Laboratory
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Mass spectrometry
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| Form |
Electronic book
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| Author |
Nair, Hari
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Clarke, William, 1970-
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| ISBN |
0128009926 |
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9780128009925 |
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