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E-book

Title Protein cages : methods and protocols / edited by Brendan P. Orner
Published New York, NY : Humana Press, [2015]
©2015

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Description 1 online resource (x, 190 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1940-6029 ; 1252
Methods in molecular biology (Clifton, N.J.) ; v. 1252. 1064-3745
Contents Encapsulation of nanoparticles in virus protein shells / Irina B. Tsvetkova and Bogdan G. Dragnea -- Use of protein cages as a template for confined synthesis of inorganic and organic nanoparticles / Masaki Uchida [and three others] -- Ferritin encapsulation and templated synthesis of inorganic nanoparticles / Katherine W. Pulsipher and Ivan J. Dmochowski -- Determining the relaxivity values of protein cage-templated nanoparticles using magnetic resonance imaging / Barindra Sana and Sierin Lim -- Computationally assisted engineering of protein cages / Maziar S. Andrejani and Brendan P. Orner -- Recombinant expression and purification of "virus-like" bacterial encapsulin protein cages / W. Frederik Rurup, Jeroen J.L.M. Cornelissen, and Melisa S.T. Koay -- Production of bacterial microcompartments / Jonathan K. Lassila -- Detectiopn of protein cage assembly with bisarsenic fluorescent probes / Thomas A. Cornell and Brendan P. Orner -- Determining the role of metal binding in protein cage assembly / Anne Grove, Ambuj K. Kushwaha, and Khoa H. Nguyen -- Differential scanning calorimetry to quantify the stability of protein cages / Yu Zhang and Maziar S. Ardejani -- Material properties of viral nanocages explored by atomic force microscopy / Mariska G.M. van Rosmalen, Wouter H. Roos, and Gijs J.L. Wuite -- Computational mechanics of viral capsids / Melissa G. Gibbons, Luigi E. Perotti, and William S. Klug
Summary This volume emphasizes new techniques to help understand protein cages and to apply them to a variety of technologies, highlighting the expertise of researchers based on three continents. Protein cages are currently inspiring diverse scientific disciplines and are therefore at the crossroads of extremely widely-scoped research, which is reflected in the detailed chapters of Protein Cages: Methods and Protocols. From nanomaterials studies and iron particles to computational strategies and Atomic Force Microscopy, the chapters herein collectively provide an introduction to the rich world of protein cage research and specific techniques to understand and exploit this fascinating class of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters begin with an introduction to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Protein Cages: Methods and Protocols will help to inspire and further propel the current multi-disciplinary enthusiasm in studying and discovering new applications for protein cages
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed November 24, 2014)
Subject Proteins -- Laboratory manuals
Carrier proteins.
Proteins -- Conformation.
Proteins -- chemistry
Nanostructures -- chemistry
Nanotechnology -- methods
Carrier Proteins
Protein Conformation
Materials science.
Proteins.
Science -- Life Sciences -- Biochemistry.
Technology & Engineering -- Material Science.
Proteins -- Conformation
Carrier proteins
Proteins
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Orner, Brendan P., editor.
ISBN 9781493921317
1493921312