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Book Cover
E-book
Author Panconi, Luca

Title Methods for Imaging Cell Membranes
Published Milton : Taylor & Francis Group, 2023

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Description 1 online resource (137 p.)
Series Series in Cellular and Clinical Imaging Series
Series in Cellular and Clinical Imaging Series
Contents 2.2.4 Imaging Hardware -- 2.2.5 3D Methods -- 2.3 Image Processing -- 2.3.1 Filtering -- 2.3.2 Drift Correction -- 2.3.3 Localisation -- 2.3.4 Blink Correction -- 2.4 Image Quality Metrics -- 2.4.1 Density and Localisation Precision -- 2.4.2 Fourier Methods and Resolution -- 2.4.3 Artefact Detection -- 2.5 Potential Image Artefacts -- 2.5.1 Multiple Blinking -- 2.5.2 Localisation Precision -- 2.5.3 Drift -- 2.5.4 Over-Density -- 2.6 Quantification -- 2.6.1 The Nature of SMLM Data -- 2.6.2 Cluster Analysis -- 2.6.3 Choices of Algorithms -- 2.7 Biological Examples -- 2.7.1 Neurons
3.2.4 Comparison and Limitations of FCS, FRAP, and SMT -- 3.3 Using FCS to Measure Diffusion of Biomolecules -- 3.3.1 Hardware -- 3.3.2 Fluorescent Labels for FCS -- 3.3.3 FCS Data and Processing -- 3.4 Using SMT to Measure Diffusion of Biomolecules -- 3.4.1 Hardware -- 3.4.2 Fluorescent Labels for SMT -- Conjugation and Properties -- 3.4.3 SMT Data and Processing -- 3.5 Advances and Extensions of FCS and SPT -- 3.5.1 Fluorescence Imaging Correlation Spectroscopies -- 3.5.2 Super-Resolution FCS -- 3.5.3 SMT at High Molecular Concentrations
Notes Description based upon print version of record
3.6 Insights into Biomolecular Regulation and Behaviour Using FCS and SPT
Form Electronic book
Author Nieves, Daniel
Makarova, Maria
Owen, Dylan
ISBN 9781003823148
1003823149