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Title Nuclear receptors : a practical approach / edited by Didier Picard
Published New York : Oxford University Press, 1999
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Description 1 online resource (xviii, 285 pages) : illustrations (some color)
Series Practical approach series ; 207
Practical approach series ; 207
Contents Machine generated contents note: 1. Evolutionary biology of the nuclear receptor superfamily / M. Robinson / H. Escriva / V. Laudet -- 2. Initial characterization of new orphan receptors / R. Sladek / V. Giguere -- 3. Adopting orphans: finding ligands / W. Wahli / S. Kersten -- 4. Kinetic analysis of nuclear receptor interactions / L. P. Freedman / B. J. Cheskis -- 5. Functional characterization of co-activators using mammalian cell microinjection / T.-M. Mullen / M. G. Rosenfeld / [et al.] / D. W. Rose -- 6. Analysis of steroid/nuclear receptor phosphorylation / N. L. Weigel / B. G. Rowan -- 7. Ligand- and cofactor-regulated transcription with chromatin templates / W. L. Kraus / J. T. Kadonaga -- 8. Hormone-resistance syndromes / M. Gurnell / V. K. K. Chatterjee -- 9. In vitro assembly of steroid receptor -- Hsp90 complexes / D. F. Smith -- 10. Yeast as a system for the study of nuclear receptor function / M. J. Garabedian / J. A. Iniguez-Lluhi / A. Kralli -- 11. Regulation of heterologous proteins by fusion to a hormone binding domain / D. Picard
Summary The steroid / nuclear receptor superfamily is a large and growing group of transcription factors that are studied by a large and varied number of basic and clinical researchers. The first two chapters describe the evolutionary biology of the superfamily and explain how to clone and characterize new receptors. Chapter 3 shows how to identify the ligands of novel receptors and chapter 4 explains the kinetic analysis of receptor interactions. In chapter 5, the reader is guided through the functional characterization of coactivators using microinjection. The next section covers receptor phosphorylation, ligand regulated transcription, and hormone resistance syndromes. Chapter 9 describes the in vitro assembly of Hsp90 complexes and chapter 10 explains yeast as a model system for looking at receptor function. The final chapter shows how heterologous proteins can be regulated by fusion to the hormone binding domain of a receptor. Nuclear Receptors: A Practical Approach is a comprehensive guide to studying members of the superfamily and will be invaluable to all researchers old and new
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Nuclear receptors (Biochemistry)
Steroid hormones -- Receptors.
Transcription factors.
Receptors, Cytoplasmic and Nuclear.
Transcription Factors.
Receptors, Steroid.
Genre/Form Laboratory Manual.
Laboratory manuals.
Form Electronic book
Author Picard, Didier.
ISBN 0585483728 (electronic bk.)
1280375698
9780585483726 (electronic bk.)
9781280375699