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Author Pisapati, Avani V

Title Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  


Description online resource (streaming video file)(527 seconds)
Series Bioengineering
Summary We present a protocol for immobilizing single macromolecules in microfluidic devices and quantifying changes in their conformations under shear flow. This protocol is useful for characterizing the biomechanical and functional properties of biomolecules such as proteins and DNA in a flow environment
Notes Title from resource description page
Audience For undergraduate, graduate, and professional students
Notes English
Subject Bioengineering
Form Streaming video