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Author Portolano, Nicola

Title Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  


Description online resource (streaming video file)(680 seconds)
Series Biology
Summary The expression of recombinant proteins by mammalian systems is becoming an attractive method for producing protein complexes for structural biology. Here we present a simple yet highly efficient expression system using suspension grown mammalian cells to purify protein complexes for structural studies
Notes Title from resource description page
Audience For undergraduate, graduate, and professional students
Notes English
Subject Biology
Form Streaming video