Limit search to available items
Book Cover
E-book
Author Rashid, A.

Title Cell Physiology and Genetics of Higher Plants : Volume II
Published Milton : CRC Press, 2018
Online access available from:
ProQuest Ebook Central    View Resource Record  

Copies

Description 1 online resource (272 pages)
Contents Cover; Title Page; Copyright Page; Preface; The Author; Cell Physiology and Genetics of Higher Plants; Contents; Chapter 1 Protoplast â#x80;#x94; Isolation and Cell Regeneration; I. Definition and Significance; II. Isolation; A. Methods of Isolation; 1. Mechanical Isolation; 2. Enzymatic Isolation; a. Steps in Enzymatic Isolation; B. Factors Affecting Isolation; 1. Source Material; a. Mesophyll Tissue; b. Callus and Cell Suspension; c. Pollen Grains; 2. Osmoticum; 3. Enzymes; 4. Isolation Medium; 5. Incubation Conditions; 6. Tissue Pretreatment; III. Harvest, Purification, and Viability
B. Protoplast Culture of Monocot PlantsIX. Protoplast to Plant; X. Microplasts/Cytoplasts and their Utility; XI. Protoplast â#x80;#x94; Applications in Fundamental Research; A. Isolation of Cell Components; B. Photosynthesis; C. Stomatal Physiology; D. Environmental Monitoring; E. Cell Wall Formation; F. Herbicide Physiology; G. Circadian Rhythm; H. Nitrogen Metabolism; I. Hormone Physiology; References; 2. Cell Modification; I. Introduction; II. Cell Genetics â#x80;#x94; Complementary to Conventional Genetics; III. Terminology â#x80;#x94; Mutant vs. Variant; A. Epigenetic Variation; B. Mutation
C. Limits to SelectionVII. System; A. Necessity of Plating Technique; B. Mutagen Treatment; C. Mutation Frequency; D. Ploidy of Plant Cells; E. Model System; VIII. Variant Cell Lines; A. Pigment Variants; B. Alkaloid Variants; C. Antibiotic Resistance; D. Resistance to Purine and Pyrimidine Analogues; E. Resistance to Amino Acid Analogues; F. Resistance to Toxins; G. Resistance to Herbicides; H. Resistance to Stress; 1. Chilling Stress; 2. Heat Stress; 3. Salt Stress; 4. Drought Stress; 5. Metal Stress; I. Developmental Variants; 1. Growth Regulator Variants; 2. Transcriptional Variants
IV. Protoplast to Cell â#x80;#x94; A TransitionV. Culture; A. Methods of Culture; 1. Suspension Culture; a. Single Drop or Multiple Drops; b. Microchamber Culture; c. Microdroplet Culture; 2. Plating of Protoplasts; VI. Cell Regeneration; A. Cell Wall Formation; B. Cell Division; C. Factors Affecting Division; 1. Physical Environment; a. Density; b. Light; c. Temperature; 2. Chemical Environment; a. Nutrient Medium; b. Osmoticum and Carbon Source; c. Hormones and Growth Regulators; d. pH; 3. Genetic Nature; VII. Culture at Low Density; VIII. Problems in Protoplast Culture; A. General
IV. Spontaneous Modification or Somaclonal VariationA. Variant Plants with Desirable Characters; 1. Sugarcane; 2. Potato; 3. Maize; 4. Alfalfa; 5. Geranium; 6. Brassica; B. Variant Plants with New Characters; 1. Tobacco; 2. Rice; 3. Wheat; 4. Tomato; C. Variants with Chromosome Modifications; D. Nature of Variation; 1. Is Variation Preexisting?; 2. Is Variation Simple or Complex?; 3. Is Variation Heritable?; E. Origin of Variation; F. Limitations and Prospects; V. Induced Modification; VI. Selection; A. Direct Selection for Resistance; B. Indirect Selection for Conditional Mutants
Notes J. Metabolic Variants
Print version record
Subject Plant cells and tissues.
Plant cytogenetics.
Plant cells and tissues.
Plant cytogenetics.
Form Electronic book
ISBN 1351087371
9781351087377