Title Enzyme engineering : methods and protocols / edited by James C. Samuelson

Published New York : Humana Press : Springer, ©2013

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Description 1 online resource (xi, 252 pages) : illustrations (some color) Series Methods in molecular biology, 1940-6029 ; v. 978 Methods in molecular biology (Clifton, N.J.) ; v. 978. 1064-3745 Contents A Tripartite fusion system for the selection of protein variants with increased stability in vivo / Linda Foit and James C.A. Bardwell -- Determining enzyme kinetics via isothermal titration calorimetry / Neil A. Demarse, Marie C. Killian, Lee D. Hansen, Colette F. Quinn -- GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria / Olga Paley, Giulia Agnello, Jason Cantor, Tae Hyun Yoo -- Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties / Sarah K. Baxter, Abigail R. Lambert, Andrew M. Scharenberg -- TAL effector nuclease (TALEN) engineering / Ting Li and Bing Yang -- In vitro evolution of enzymes / Misha V. Golynskiy, John C. Haugner III, Aleardo Morelli -- Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo / Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington -- Reconstructing evolutionary adaptive paths for protein engineering / Megan F. Cole, Vanessa E. Cox, Kelsey L. Gratton, Eric A. Gaucher -- Oligonucleotide recombination enabled site-specific mutagenesis in bacteria / Bryan M. Swingle -- FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants / Eric R. Geertsma -- Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes / Hidehiko Hirakawa and Teruyuki Nagamune -- Gene synthesis by assembly of deoxyuridine-containing oligonucleotides / Romualdas Vaisvila and Jurate Bitinaite -- Protein engineering : single or multiple site-directed mutagenesis / Pei-Chung Hsieh and Romualdas Vaisvila -- Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination / Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish -- Promiscuity-based enzyme selection for rational directed evolution experiments / Sandeep Chakraborty, Renu Minda, Lipika Salaye, Abhaya M. Dandekar -- Rational protein sequence diversification by multi-codon scanning mutagenesis / Jia Liu and T. Ashton Cropp -- Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter / Jian-Wei Liu and David L. Ollis -- In vitro directed evolution of enzymes expressed by E. coli in microtiter plates / Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis Summary Whether the pursuit is commercially motivated or purely academic, engineering a novel biological catalyst is an enticing challenge. High-resolution protein structure analysis allows for rational alteration of enzyme function, yet many useful enzyme variants are the product of well-designed selection schemes or screening strategies. Enzyme Engineering: Methods and Protocols provides guidance to investigators wishing to create enzyme variants with desired properties. This detailed volume covers such topics as a simple method for generating site-specific mutations within bacterial chromosomes. It also highlights the engineering of two difference types of rare-cutting endonucleases that show great potential in gene therapy applications: The newest development is the emergence of TAL effector nucleases or TALENs. Chapters describe newly developed technologies in sufficient detail so that each method can be practiced in a standard molecular biology laboratory. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible Enzyme Engineering: Methods and Protocols will be valuable for scientists with a budding interest in protein engineering as well as veterans looking for new approaches to apply in established discovery programs Analysis enzymen enzymes biochemie biochemistry enzymologie enzymology protocollen protocols Proteins and Enzymes Eiwitten en enzymen Bibliography Includes bibliographical references and index Notes English Print version record Subject Enzymes -- Biotechnology Enzymes -- Industrial applications. Protein engineering. Engineering. Medical personnel. Genetic engineering. Genetics -- Technique. Enzymes. Biomedical engineering. Methodology. Enzymes and Coenzymes Engineering Investigative Techniques Health Occupations Genetic Engineering Publication Formats Publication Characteristics Chemicals and Drugs Technology, Industry, and Agriculture Disciplines and Occupations Analytical, Diagnostic and Therapeutic Techniques and Equipment Genetic Techniques Technology, Industry, Agriculture Enzymes Laboratory Manuals Biomedical Engineering Methods Protein Engineering engineering. enzyme. biomedical engineering. methodology. Methodology Medical personnel Genetics -- Technique Genetic engineering Enzymes Engineering Biomedical engineering Enzymes -- Biotechnology Enzymes -- Industrial applications Protein engineering Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Samuelson, James C., 1968- ISBN 9781627032933 1627032932

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