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Title Protein terminal profiling : methods and protocols / edited by Oliver Schilling
Published New York, N.Y. : Humana Press : Springer, [2017]
©2017

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Description 1 online resource (xii, 268 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1574
Methods in molecular biology (Clifton, N.J.) ; v. 1574. 1064-3745
Contents [14C]-Acetyl-Coenzyme A-Based in vitro N-Terminal acetylation assay / Adrian Drazic and Thomas Arnesen -- DTNB-based quantification of in vitro enzymatic N-Terminal acetyltransferase activity / Håvard Foyn, Paul R. Thompson, and Thomas Arnesen -- SILProNAQ : a convenient approach for proteome-wide analysis of protein N-Termini and N-Terminal acetylation quantitation / Willy V. Bienvenut, Carmela Giglione, and Thierry Meinnel -- Profiling of protein N-Termini and their modifications in complex samples / Fatih Demir, Stefan Niedermaier, Jayachandran N. Kizhakkedathu, and Pitter F. Huesgen -- Protease substrate profiling by N-Terminal COFRADIC / An Staes, Petra Van Damme, Evy Timmerman, Bart Ruttens, Elisabeth Stes, Kris Gevaert, and Francis Impens -- Doublet N-Terminal oriented proteomics for N-Terminomics and proteolytic processing identification / Benoit Westermann, Alvaro Sebastian Vaca Jacome, Magali Rompais, Christine Carapito, and Christine Schaeffer-Reiss -- Multidimensional analysis of protease substrates and their cellular origins in mixed secretomes from multiple cell types / Pascal Schlage and Ulrich auf dem Keller -- System-wide profiling of protein amino termini from formalin-fixed, paraffin-embedded tissue specimens for the identification of novel substrates / Zon W. Lai and Oliver Schilling -- Identification of carboxypeptidase substrates by C-Terminal COFRADIC / Sebastian Tanco, Francesc Xavier Aviles, Kris Gevaert, Julia Lorenzo, and Petra Van Damme -- ProC-TEL : profiling of protein C-Termini by enzymatic labeling / Wenwen Duan and Guoqiang Xu -- Determining protease substrates within a complex protein background using the PROtein TOpography and migration analysis platform (PROTOMAP) / R.A. Fuhrman-Luck, L.M. Silva, M.L. Hastie, J.J. Gorman, and J.A. Clements -- Multiplexed Protease Specificity Profiling Using Isobaric Labeling / Joanna Tucher and Andreas Tholey -- FPPS : fast profiling of protease specificity / Matej Vizovišek, Robert Vidmar, and Marko Fonović -- Profiling of protease cleavage sites by proteome-derived peptide libraries and quantitative proteomics / Chia-yi Chen, Bettina Mayer, and Oliver Schilling -- Prediction of proteases involved in peptide generation / Mercedes Arguello Casteleiro, Robert Stevens, and Julie Klein -- Live-cell imaging of protease activity : assays to screen therapeutic approaches / Anita Chalasani, Kyungmin Ji, Mansoureh Sameni, Samia H. Mazumder, Yong Xu, Kamiar Moin, and Bonnie F. Sloane -- Protein translocation assays to probe protease function and screen for inhibitors / Angelina Hahlbrock, Dorothée Gösswein, and Roland H. Stauber -- Simultaneous detection of metalloprotease activities in complex biological samples using the PrAMA (proteolytic activity matrix assay) method / Catharina Conrad, Miles A. Miller, Jörg W. Bartsch, Uwe Schlomann, and Douglas A. Lauffenburger -- Synthesis and application of activity-based probes for proteases / Tim Van Kersavond, Minh T.N. Nguyen, and Steven H.L. Verhelst
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed March 21, 2017)
Subject Proteins.
Proteolysis.
Proteolysis
Protein Interaction Mapping -- methods
N-Terminal Acetyltransferases
Peptide Hydrolases
Proteins
protein.
Proteins.
Science -- Life Sciences -- Biochemistry.
Proteolysis
Proteins
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Schilling, Oliver, 1975- editor.
ISBN 9781493968503
1493968505