| Description |
1 online resource (x, 168 pages) : illustrations |
| Series |
Methods in molecular biology, 1064-3745 ; v. 258 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 258
|
| Contents |
Technical considerations in quantitating gene expression / R.A. Shimkets -- Gene expression quantitation technology summary / R.A. Shimkets -- Standardized RT-PCR and the standardized expression measurement center / J.C. Willey [and others] -- Genecalling: Transcript profiling coupled to a gene database query / R.A. Shimkets -- Invader assay for rna quantitation / M.C. Olson [and others] -- Monitoring eukaryotic gene expression using oligonucleotide microarrays / J. Lescallett [and others] -- Amplified differential gene expression microarray / Z.J. Chen and K.D. Tew -- Suppression subtractive hybridization / D.V. Rebrikov [and others] -- Small amplified rna-sage / C. Vilain and G. Vassart -- Gene expression informatics / M. Leach |
| Summary |
The transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In Gene Expression Profiling: Methods and Protocols, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and tissues. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Special care has been taken to distill the basic underlying principles of each method to a few straightforward concepts so that investigators can easily choose the method most appropriate to their application. The readily reproducible techniques presented include new methods for applying the GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. Cutting-edge and highly practical, Gene Expression Profiling: Methods and Protocols allows novice and experienced investigators alike not only to choose the best method to determine how genes are turned on and off in many different organisms, but also to use informatics resources to analyze and understand the results |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Gene expression -- Laboratory manuals
|
|
Gene Expression Profiling -- methods
|
|
SCIENCE -- Life Sciences -- Genetics & Genomics.
|
|
Gene expression
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Shimkets, Richard A
|
| ISBN |
1592597513 |
|
9781592597512 |
|
1588292207 |
|
9781588292209 |
|
