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Title Handbook of cryo-preparation methods for electron microscopy / edited by Annie Cavalier, Daniele Spehner, Bruno M. Humbel
Published Boca Raton : CRC Press, [2009]
Online access available from:
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Description 1 online resource (xix, 682 pages) : illustrations
Series Methods in visualization
Methods in visualization.
Contents Slam-freezing, metal-mirror freezing / Daniele Spehner and Ludwig Edelmann -- Plunge-freezing (Holey carbon method) / Sacha De Carlo -- Controlled vitrification / Peter M. Frederik, Felix de Haas, and Marc M.H. Storms -- BAL-TEC HPM 010 high-pressure freezing machine / Andres Kaech -- High-pressure freezing Leica EMPACT / Dimitri Vanhecke and Daniel Studer -- Frozen-hydrated macromolecules for structural analysis / Corinne Crucifix, Gabor Papai and Patrick Schultz -- Two-dimensional crystals / Patrick Bron and Janet Vonck -- Cryo-negative staining / Sacha De Carlo -- Vitrification of dynamic microtubules / Isabelle Arnal, Marine Malleter, and Denis Chretien -- CEMOVIS : cryo-electron microscopy of vitreous sections / Jacques Dubochet [and others] -- Cryo-electron tomography / Andrew Leis, Manuela Gruska, and Wolfgang Baumeister -- Freeze-substitution / Bruno M. Humbel -- Cryo-fixation, freeze-substitution, rehydration and Tokuyasu cryo-sectioning / York-Dieter Stierhof [and others] -- Freeze-drying and embedding of biological material / Ludwig Edelmann -- The shadow of hydrated biological specimens / Daniel Thomas -- Cryo-fracture of self assembled systems in organic solvent / Marc Schmutz and Philippe J. Mesini -- Progressive lowering of temperature for immunolabeling and in situ hybridization / Annie Cavalier and Daniele Spehner -- Cryo-sectioning according to Tokuyasu / Bruno M. Humbel and York-Dieter Stierhof -- Cryo-preparation procedures for elemental imaging by SIMS and EFTEM / Christian Bordat and Jean-luc Guerquin-Kern -- Correlative light and electron microscopy / Heinz Schwarz and Bruno M. Humbel -- SDS-digested freeze-fracture replica labeling (SDS-FRL) / Yugo Fukazawa [and others] -- Immunolabeling of ultrathin sections with enlarged 1 nm gold or QDots / York-Dieter Stierhof -- 3-D electron tomography of cells and organelles / Willie J.C. Geerts, Bruno M. Humbel and Arie J. Verkleij -- Final considerations / Hellmuth Sitte
Bibliography Includes bibliographical references and index
Subject Cryobiology -- Methodology.
Electron microscopy -- Methodology.
Cryopreservation -- methods.
Microscopy, Electron -- methods.
Genre/Form Laboratory Manual.
Form Electronic book
Author Cavalier, Annie.
Humbel, Bruno M.
Spehner, Danièle.
ISBN 0849372275 (hardcover ; alk. paper)
9780849372278 (hardcover ; alk. paper)