Description |
xvii, 230 pages : illustrations |
Contents |
Looking at yeast cells -- Isolation and characterization of auxotrophic, temperature-sensitive, and osmotic-sensitive mutants -- Meiotic mapping -- Mitotic recombination and random spore analysis -- Transformation of yeast -- Synthetic lethal mutants -- Gene replacement -- Isolation of ras2 suppressors -- Manipulating cell types -- Isolating mutants by insertional mutagenesis -- Isolation of separation of function mutants by two-hybrid differential interaction screening -- High-efficiency transformation of yeast -- "Quick and dirty" plasmid transformation of yeast colonies -- Yeast DNA isolations -- Yeast protein extracts -- TAP purification -- Yeast RNA isolations -- Hydroxylamine mutagenesis of plasmid DNA -- Assay of beta-galactosidase in yeast -- Plate assay for carboxypeptidase Y -- Random spore analysis -- Yeast vital stains -- Yeast immunofluorescence -- Actin staining in fixed cells -- PCR-mediated gene disruption -- Yeast colony PCR -- Measuring yeast cell density by spectrophotometry -- Cell synchrony -- Chromatin immunoprecipitation -- Flow cytometry of yeast DNA -- Logarithmic growth -- EMS mutagenesis -- Tetrad dissection -- Making a tetrad dissection needle -- Picking zygotes -- Determining plating efficiency -- DNA miniprep from e. coli -- Preparing and transforming competent e. coli -- Storing and handling the systematic deletion collection -- Media -- Stock preservation -- Yeast genetic and physical maps -- Templates for making streak plates -- Electrophoretic karyotypes of strains for southern blot mapping -- Strains -- Counting yeast cells with a standard hemocytometerchamber -- Tetrad scoring sheet |
Notes |
Rev. ed. of: Methods in yeast genetics / Dan Burke, Dean Dawson, Tim Stearns, 2000 ed. 2000 |
Bibliography |
Includes bibliographical references |
Author |
Strathern, Jeffrey N.
|
|
Burke, Dan, 1954-
|
|
Amberg, David C.
|
|
Burke, Dan, 1954-
Methods in yeast genetics
|
|
Cold Spring Harbor Laboratory.
|
LC no. |
2004028765 |
ISBN |
0879697288 paperback |
|