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Author Waikagul, Jitra.

Title Approaches to Research on the Systematics of Fish-Borne Trematodes
Published Burlington : Elsevier Science, 2014
Online access available from:
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Description 1 online resource (119 pages)
Contents Front Cover; Approaches to Research on the Systematics of Fish-Borne Trematodes; Copyright Page; Contents; Preface; 1 Medically Important Fish-Borne Zoonotic Trematodes; 1.1 Major fish-borne Trematodes; 1.1.1 Opisthorchiidae; 1.1.2 Treatment of Liver Flukes; 1.2 Other fish-borne Trematodes; 1.2.1 Clinostomatidae; 1.2.2 Echinostomatidae; 1.2.3 Heterophyidae; 1.2.4 Troglotrematidae; References; 2 Collection of Fish-Borne Trematodes from the Final Host; 2.1 Diagnostic Methods; 2.1.1 Stool Egg Examination; 2.1.2 Copro-DNA Detection; 2.1.3 Antibody Detection; 2.1.4 Worm Finding
1.1 Preservation of Tissue Sample in Ethanol
2.2 Treatment and Purgation2.3 Collection of Adult FBT from Animals; 2.4 Worm Preparation for Morphological Study; 2.5 Diagnostic Characteristics of FBT; 2.5.1 Family Clinostomatidae Luhe, 1901; 2.5.2 Family Echinostomatidae Looss, 1902; 2.5.3 Family Heterophyidae Leiper, 1909; 2.5.4 Family Opisthorchiidae Braun, 1901; 2.5.5 Family Troglotrematidae Odhner, 1914; References; 3 Collection of Fish-Borne Trematode Cercaria; 3.1 Snail Host of Fish-Borne Trematodes; 3.1.1 Family Bithyniidae; 3.1.2 Family Planorbidae; 3.1.3 Family Thiaridae; 3.1.4 Family Semisulcospiridae
3.2 Cercaria Morphological Study3.3 Morphology of Fresh Cercaria; 3.4 Vital Stain; 3.5 Chaetotaxy; 3.6 Polymerase Chain Reaction; References; 4 Collection of Fish-Borne Trematodes in Infective Stage from the Fish: The Second Intermediate Host; 4.1 Development of Metacercariae; 4.2 Methods of Fish Examination for Metacercariae; 4.2.1 Compression Method; 4.2.2 Digestion Method; 4.3 Mobile Laboratory for Fish Examination; 4.4 Method of Excysting Metacercariae; 4.5 Morphology of Metacercariae; 4.5.1 Clinostomatidae; 4.5.2 Echinostomatidae; 4.5.3 Heterophyidae; 4.5.4 Opisthorchiidae Life Span of Metacercariae4.5.4.2 Infectivity of Metacercariae; 4.5.5 Troglotrematidae; References; 5 Molecular Systematics of Fish-Borne Trematodes; 5.1 What is Molecular Systematics?; 5.2 Species Identification: Morphological and Molecular Approaches; 5.3 Advantages and Disadvantages of using DNA Sequences for Systematics; 5.4 Molecular Markers; 5.5 The Regions of DNA used for Molecular Systematics; 5.5.1 Nuclear Coding Region; 5.5.2 Ribosomal DNA; 5.5.3 Mitochondrial DNA; 5.6 Molecular Systematics of fish-borne Trematodes
5.6.1 Opisthorchiidae Looss 1899 and Heterophyidae Leiper 19095.6.2 Clinostomidae Lühe, 1901; 5.7 Summary and Future Direction; References; 6 Methods of Molecular Study: DNA Sequence and Phylogenetic Analyses; 6.1 Criterion for Selecting Appropriate Genetic Markers; 6.2 Phylogenetic Analysis; 6.3 How to Construct Phylogenetic Trees; 6.3.1 Assembling a Dataset; 6.3.2 Multiple Sequence Alignment; 6.3.3 Determination of Substitution Model; 6.3.4 Tree Building; 6.3.5 Tree Evaluation; 6.4 Further Perspectives; References; Appendix A; References; Appendix B; 1 Genomic DNA Preparation
Summary Approaches to Research on the Systematics of Fish-Borne Trematodes is a concise guide for systematic studies of the prevalence of fish-borne trematodes both in the endemic areas and experimental laboratories. It includes methods to identify species of fish-borne trematodes to enhance the precision of research studies based on the metacercarial stage. Misidentification of trematode species is a common occurrence when researchers are new to the field and have no guidance. Consequentially, sometimes publications report inaccurate prevalence rates of these parasites. This compact guide gives cl
Notes Description based upon print version of record
Form Electronic book
Author Thaekham, Urusa
ISBN 9780124078604