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Author Wei, Chin-Wen

Title Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
Published Cambridge, MA MyJoVE Corp 2016
Online access available from:
Journal of Visualized Experiments    View Resource Record  

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Description 1 online resource (streaming video file) (382 secs.)
Series Immunology and Infection
Summary This article illustrates a powerful method to quantify mitochondria or lysosomes in living cells. The combination of lysosome- or mitochondria-specific dyes with fluorescently conjugated antibodies against surface markers allows the quantification of these organelles in mixed cell populations, like primary cells harvested from tissue samples, by using multicolor flow cytometry
Audience For undergraduate, graduate, and professional students
Notes English
Description based on online resource; title from resource description page
Subject Immunology and Infection
Form Streaming audio