Title MTor : methods and protocols / edited by Thomas Weichhart

Published New York : Humana Press : Springer, ©2012

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Description 1 online resource (xiii, 464 pages) : illustrations Series Methods in molecular biology ; vol. 821 Methods in molecular biology (Clifton, N.J.) ; v. 821 Contents Mammalian target of rapamycin : a signaling kinase for every aspect of cellular life / Thomas Weichhart -- Biochemical and pharmacological inhibition of mTOR by rapamycin and an ATP-competitive mTOR inhibitor / Ker Yu and Lourdes Toral-Barza -- Evaluation of the nutrient-sensing mTOR pathway / Sungki Hong, Aristotle M. Mannan, and Ken Inoki -- mTor activity under hypoxia / Douangsone D. Vadysirisack and Leif W. Ellisen -- Isolation of the mTOR complexes by affinity purification / Dos D. Sarbassov [and others] -- An in vitro assay for the kinase activity of mTOR complex 2 / Jingxiang Huang -- Overexpression or downregulation of mTOR in mammalian cells / Mahmoud Khalil and Ivan Gout -- Detection of cytoplasmic and nuclear functions of mTOR by fractionation / Margit Rosner and Markus Hengstschläger -- Evaluation of rapamycin-induced cell death / Lorenzo Galluzzi [and others] -- Evaluation of mTOR-regulated mRNA translation / Valentina Iadevaia [and others] -- A genome-wide RNAi screen for polypeptides that alter rpS6 phosphorylation / Angela Papageorgiou and Joseph Avruch -- Immunohistochemical analysis of mTOR activity in tissues / Jinhee Kim [and others] -- Assessing cell size and cell cycle regulation in cells with altered TOR activity / Megan Cully and Julian Downward -- Quantitative visualization of autophagy induction by mTOR inhibitors / Beat Nyfeler [and others] -- The in vivo evaluation of active-site TOR inhibitors in models of BCR-ABL+ leukemia / Matthew R. Janes and David A. Fruman -- Inducible raptor and rictor knockout mouse embryonic fibroblasts / Nadine Cybulski, Vittoria Zinzalla, and Michael N. Hall -- Expanding human T regulatory cells with the mTOR-inhibitor rapamycin / Manuela Battaglia, Angela Stabilini, and Eleonora Tresoldi -- Rapamycin-induced enhancement of vaccine efficacy in mice / Chinnaswamy Jagannath and Pearl Bakhru -- Utilizing a retroviral RNAi system to investigate in vivo mTOR functions in T cells / Koichi Araki and Bogumila T. Konieczny -- Exploring functional in vivo consequences of the selective genetic ablation of mTOR signaling in T helper lymphocytes / Greg M. Delgoffe and Jonathan D. Powell -- Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics / Huawei Li, Jennifer L. Cotton, and David A. Guertin -- Inhibition of PI3K-Akt-mTOR signaling in glioblastoma by mTORC1/2 inhibitors / Qi-Wen Fan and William A. Weiss -- Assessing the function of mTOR in human embryonic stem cells / Jiaxi Zhou, Dong Li, and Fei Wang -- Video-EEG monitoring methods for characterizing rodent models of tuberous sclerosis and epilepsy / Nicholas R. Rensing, Dongjun Guo, and Michael Wong -- A genetic model to dissect the role of TSC-mTORC1 in neuronal cultures / Duyu Nie and Mustafa Sahin -- Tissue-specific ablation of TSC1 in pancreatic beta-cells / Hiroyuki Mori and Kun-Liang Guan -- A mouse model of diet-induced obesity and insulin resistance / Chao-Yung Wang and James K. Liao -- Rapamycin as immunosuppressant in murine transplantation model / Louis-Marie Charbonnier and Alain Le Moine -- Development of ATP-competitive mTOR inhibitors / Qingsong Liu [and others] Summary Since its discovery, the mammalian target of rapamycin (mTOR) has been shown to regulate many critical molecular processes in eukaryotes such as metabolism, growth, survival, aging, synaptic plasticity, memory, and immunity. In mTOR: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study mTOR. These include methods and techniques used for the study of the mTOR pathway and potential therapeutic applications of mTOR inhibitors such as, immunosuppressive and anticancer agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, mTOR: Methods and Protocols seeks to aid scientists in the further study of this crucially important molecule ranging from the analysis of signal transduction events within a cell to the assessment of complex human diseases such as metabolic disorders or cancer Analysis celbiologie cellular biology moleculaire biologie molecular biology immunologie immunology metabolisme metabolism Cellular Biology Celbiologie Bibliography Includes bibliographical reference and index Notes Print version record Subject Rapamycin -- Laboratory manuals TOR Serine-Threonine Kinases Rapamycin Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Weichhart, Thomas ISBN 9781617794308 1617794309

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