| Description |
1 online resource (xxx, 530 pages) : illustrations |
| Contents |
Front Cover; Contents; Preface; Authors; Chapter 1: Strategies for Novel Research Projects and/or Research Grant Funding; Chapter 2: Rapid Isolation of Specific cDNAs or Genes by PCR; Chapter 3: Construction and Screening of Subtracted and Complete Expression cDNA Libraries; Chapter 4: Subcloning of Genes or DNA Fragments; Chapter 5: Nonisotopic and Isotopic DNA or RNA Sequencing; Chapter 6: Bioinformation Superhighway and Computer Databases of Nucleic Acids and Proteins; Chapter 7: Characterization of DNA or Genes by Southern Blot Hybridization |
| Summary |
"The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. Other topics include approaches to grant funding and SiRNA technology. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. This edition features new techniques for every chapter, as well as several new chapters"--Provided by publisher |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Genetic engineering -- Laboratory manuals
|
|
Molecular biology -- Laboratory manuals
|
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Genetic Engineering -- methods
|
|
Biotechnology -- methods
|
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Genetic engineering
|
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Molecular biology
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Zhang, Helen H., author
|
|
Welsh, Michael J., author
|
|
Kaufman, Peter B., author
|
| LC no. |
2010051443 |
| ISBN |
1439848327 |
|
9781439848326 |
|
1439848300 |
|
9781439848302 |
|
