| Description |
1 online resource (xi, 274 pages) : illustrations (some color) |
| Series |
Springer protocols |
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Methods in molecular biology, 1940-6029 ; v. 1503 |
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Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 1503
|
| Contents |
Ubiquitous importance of protein glycosylation / Jasminka Krištić and Gordan Lauc -- Laboratory experimental design for a glycomic study / Ivo Ugrina, Harry Campbell, and Frano Vučković -- High-throughput analysis of the IgG N-glycome by UPLC-FLR / Maja Pučić-Baković -- High-throughput analysis of IgG Fc glycopeptides by LC-MS / David Falck [and others] -- Sialic acid derivatization for the rapid subclass- and sialic acid linkage-specific MALDI-TOF-MS analysis of IgG Fc-glycopeptides / Noortje de Haan, Karli R. Reiding, and Manfred Wuhrer -- Use of multiple reaction monitoring on QQQ-MS for the analysis of protein- and site-specific glycosylation patterns in serum / L. Renee Ruhaak -- Analysis of permethylated glycan by liquid chromatography (LC) and mass spectrometry (MS) / Shiyue Zhou, Kerry M. Wooding, and Yehia Mechref -- High-throughput analysis of the plasma N-glycome by UHPLC / Barbara Adamczyk [and others] -- Site-specific N -- and O-glycopeptide analysis using an integrated C18-PGC-LC-ESI-QTOF-MS/MS approach / Kathrin Stavenhagen [and others] -- Analysis of milk oligosaccharides by mass spectrometry / Lauren D. Wu, L. Renee Ruhaak, and Carlito B. Lebrilla -- N -- and O-glycomics from minor amounts of formalin-fixed, paraffin-embedded tissue samples / Hannes Hinneburg [and others] -- Carbohydrate microarray technology applied to high-throughput mapping of plant cell wall glycans using comprehensive microarray polymer profiling (CoMPP) / Stjepan Krešimir Kračun [and others] -- Analysis of invertebrate and protist N-glycans / Alba Hykollari [and others] -- High-throughput and high-sensitivity mass spectrometry-based N-glycomics of mammalian cells / Stephanie Holst [and others] -- Analysis of N-glycosylation of total membrane proteins / Tamara Pavić and Olga Gornik -- HILIC-UPLC analysis of brain tissue N-glycans / Thomas Klarić and Ivan Gudelj -- Automated integration of a UPLC glycomic profile / Anna Agakova [and others] -- Databases and associated tools for glycomics and glycoproteomics / Frederique Lisacek [and others] -- High-throughput N-glycan analysis with rapid magnetic Bead-Based Sample Preparation / Marton Szigeti and Andras Guttman |
| Summary |
This volume discusses detailed protocols for the analysis of glycosylation at the level of free glycans and glycopeptides. This book covers topics such as the importance of glycans in eukaryotic life (with a focus on mammals and particularly humans); recent technologies that allow the characterization of larger sets of samples; and method repeatability and robustness, as well as higher throughput with respect to sample preparation, measurement and data analysis. The chapters spans a wide range of techniques, including the analysis of fluorescently labeled glycans with HPLC, LC-MS analysis of glycopeptides both for glycosylation profiling and for in-depth tandem mass spectrometric analysis of protein glycosylation, and the analysis of glycans with fluorescent labeling, capillary electrophoresis in conjunction with laser induced fluorescence detection. The chapters also detail specific samples types including brain tissues, N-glycans from in-vitro cell cultures, milk oligosaccharides, invertebrate and protest N-glycans, as well as plant glycans. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, High-Throughput Glycomics and Glycoproteomics: Methods and Protocols is an essential reference for researchers planning to enter this rapidly evolving field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from pdf of title page (SpringerProtocols, viewed October 17, 2016) |
| Subject |
Glycomics -- Laboratory manuals
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Proteomics -- Laboratory manuals
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High throughput screening (Drug development)
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Proteomics -- methods
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Glycomics -- methods
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Proteins.
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Science -- Life Sciences -- Biochemistry.
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Life sciences
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Biochemistry
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Glycomics
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High throughput screening (Drug development)
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Proteomics
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| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Lauc, Gordan
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Wuhrer, Manfred
|
| ISBN |
9781493964932 |
|
1493964933 |
|
1493964917 |
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9781493964918 |
|
