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Title Molecular embryology : methods and protocols / edited by Paul T. Sharpe and Ivor Mason
Published Totowa, N.J. : Humana Press, [1999]


Location Call no. Vol. Availability
Description xvi, 756 pages : illustrations ; 24 cm
Series Methods in molecular biology ; v. 97
Methods in molecular biology (Clifton, N.J.) ; v. 97
Contents Machine derived contents note: Part I. Mouse Embryo -- The Mouse as a Developmental Model -- Paul T. Sharpe -- Culture of Postimplantation Mouse Embryos -- Paul Martin and David L. Cockroft -- Organ Culture in the Analysis of Tissue Interactions -- Irma Thesleff and Carin Sahlberg -- Treatment of Mice with Retinoids In Vivo and In Vitro: Skeletal Staining -- Gillian M. Morriss-Kay -- Cell Grafting and Labeling in Postimplantation Mouse Embryos -- Gabriel A. Quinlan, Paul A. Trainor, and Patrick P. L. Tam -- Production of Transgenic Rodents by the Microinjection of Cloned DNA Into Fertilized One-Celled Eggs -- San Ling Si-Hoe and David Murphy -- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells -- Anna-Katerina Hadjantonakis, Melinda Pirity, and Andrs̀ Nagy -- Gene Trapping in Mouse Embryonic Stem Cells -- Jane Brennan and William C. Skarnes -- Production and Growth of Conditionally Immortal Cell Lines from the H-2KbtsA58 Transgenic Mouse -- Mark Noble -- Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos -- Jonathan D. Gilthorpe and Peter W. J. Rigby -- Application of lacZ Transgenic Mice to Cell Lineage Studies -- Paul A. Trainor, Sheila X. Zhou, Maala Parameswaran, Gabriel A. Quinlan, Monica Gordon, Karin Sturm, and Patrick P. L. Tam -- Mouse Primordial Germ Cells: Isolation and In Vitro Culture -- Patricia A. Labosky and Brigid L. M. Hogan -- Part II. Chicken Embryo -- The Avian Embryo: An Overview -- Ivor Mason -- Chick Embryos: Incubation and Isolation -- Ivor Mason -- New Culture -- Amata Hornbruch -- Grafting Hensen's Node -- Claudio D. Stern -- Grafting of Somites -- Claudio D. Stern -- Manipulating the Chick Embryo: Notochord Grafts -- Andrew Lumsden and Susanne Dietrich -- Transplantation of Avian Neural Tissue -- Sarah Guthrie -- Grafting of Apical Ridge and Polarizing Region -- Cheryl Tickle -- Tissue Recombinations in Collagen Gels -- Marysia Placzek and Kim Dale -- Quail-Chick Chimeras -- Marie-Aimě Teillet, Catherine Ziller, and Nicole M. Le Douarin -- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo -- Jonathan D. W. Clarke -- Part III. Amphibian Embryos -- An Overview of Xenopus Development -- C. Michael Jones and Jim Smith -- Mesoderm Induction Assays -- C. Michael Jones and Jim Smith -- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian -- Ray Keller, Ann Poznanski, and Tamira Elul -- A Method for Generating Transgenic Frog Embryos -- Enrique Amaya and Kristen L. Kroll -- Axolotl/newt -- Malcolm Maden -- Part IV. Zebrafish -- The Zebrafish: An Overview of Its Early Development -- Nigel Holder and Qiling Xu -- Small-Scale Marker-Based Screening For Mutations in Zebrafish Development -- Peter D. Currie, Thomas F. Schilling, and Philip W. Ingham -- Transgenic Zebrafish -- Trevor Jowett -- Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg For Analysis of Gene Function -- Nigel Holder and Qiling Xu -- Retinoids in Nonmammalian Embryos -- Malcolm Maden -- Part V. Nonvertebrate Chordates -- Protochordates -- Peter W. H. Holland and Hiroshi Wada -- Part VI. Retrovirus -- Gene Transfer to the Rodent Embryo by Retroviral Vectors -- Grace K. Pavlath and Marla B. Luskin -- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses -- Cairine Logan and Philippa Francis-West -- Part VII. Molecular Techniques -- Subtractive Hybridization and Construction of cDNA Libraries -- Bruce Blumberg and Juan Carlos Izpisa͠ Belmonte -- Differential Display of Eukaryotic mRNA -- Antonio Tugores and Juan Carlos Izps̕ua Belmonte -- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers -- Anthony Graham -- Single-Cell RT-PCR cDNA Subtraction -- Damian L. Weaver, Cšar Nę͠z, Clare Brunet, Victoria Bostock, and Gerard Brady -- In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections -- Radma Mahmood and Ivor Mason. In Situ Hybridization to RNA in Whole Embryos -- Huma Shamim, Radma Mahmood, and Ivor Mason -- Wholemount In Situ Hybridization to Xenopus Embryos -- C. Michael Jones and James C. Smith -- Wholemount In Situ Hybridization to Amphioxus Embryo -- Peter W. H. Holland -- In Situ Hybridization to Sections (Nonradioactive) -- Maria Rex and Paul J. Scotting -- Immunohistochemistry Using Polyester Wax -- Andrew Kent -- Immunohistochemistry on Whole Embryos -- Ivor Mason -- Whole Embryo Assays for Programmed Cell Death -- Anthony Graham -- Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos: Optimal Ring and Roller-Bottle Culture Technique -- Jonathan Cooke and Alison Isaac -- Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting -- David I. Jackson and Clive Dickson -- Part VIII. Microscopy and Photography -- Microscopy and Photomicrography Techniques -- Richard J. T. Wingate -- Index
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Also available online via the World Wide Web, by subscription to (BMP) via Humana Press
Methods in molecular biology (Clifton, N.J.) no:97 1064-3745
Subject Embryology -- Vertebrates -- Methodology
Vertebrates -- Embryology -- Methodology.
Chemical embryology -- Methodology.
Author Sharpe, Paul T.
Mason, Ivor.
Humana Press.
LC no. 98023234
ISBN 0896033872 alkaline paper