Description |
xvi, 756 pages : illustrations ; 24 cm |
Series |
Methods in molecular biology ; v. 97 |
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Methods in molecular biology (Clifton, N.J.) ; v. 97
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Contents |
Machine derived contents note: Part I. Mouse Embryo -- The Mouse as a Developmental Model -- Paul T. Sharpe -- Culture of Postimplantation Mouse Embryos -- Paul Martin and David L. Cockroft -- Organ Culture in the Analysis of Tissue Interactions -- Irma Thesleff and Carin Sahlberg -- Treatment of Mice with Retinoids In Vivo and In Vitro: Skeletal Staining -- Gillian M. Morriss-Kay -- Cell Grafting and Labeling in Postimplantation Mouse Embryos -- Gabriel A. Quinlan, Paul A. Trainor, and Patrick P. L. Tam -- Production of Transgenic Rodents by the Microinjection of Cloned DNA Into Fertilized One-Celled Eggs -- San Ling Si-Hoe and David Murphy -- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells -- Anna-Katerina Hadjantonakis, Melinda Pirity, and Andrs̀ Nagy -- Gene Trapping in Mouse Embryonic Stem Cells -- Jane Brennan and William C. Skarnes -- Production and Growth of Conditionally Immortal Cell Lines from the H-2KbtsA58 Transgenic Mouse -- Mark Noble -- Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos -- Jonathan D. Gilthorpe and Peter W. J. Rigby -- Application of lacZ Transgenic Mice to Cell Lineage Studies -- Paul A. Trainor, Sheila X. Zhou, Maala Parameswaran, Gabriel A. Quinlan, Monica Gordon, Karin Sturm, and Patrick P. L. Tam -- Mouse Primordial Germ Cells: Isolation and In Vitro Culture -- Patricia A. Labosky and Brigid L. M. Hogan -- Part II. Chicken Embryo -- The Avian Embryo: An Overview -- Ivor Mason -- Chick Embryos: Incubation and Isolation -- Ivor Mason -- New Culture -- Amata Hornbruch -- Grafting Hensen's Node -- Claudio D. Stern -- Grafting of Somites -- Claudio D. Stern -- Manipulating the Chick Embryo: Notochord Grafts -- Andrew Lumsden and Susanne Dietrich -- Transplantation of Avian Neural Tissue -- Sarah Guthrie -- Grafting of Apical Ridge and Polarizing Region -- Cheryl Tickle -- Tissue Recombinations in Collagen Gels -- Marysia Placzek and Kim Dale -- Quail-Chick Chimeras -- Marie-Aimě Teillet, Catherine Ziller, and Nicole M. Le Douarin -- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo -- Jonathan D. W. Clarke -- Part III. Amphibian Embryos -- An Overview of Xenopus Development -- C. Michael Jones and Jim Smith -- Mesoderm Induction Assays -- C. Michael Jones and Jim Smith -- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian -- Ray Keller, Ann Poznanski, and Tamira Elul -- A Method for Generating Transgenic Frog Embryos -- Enrique Amaya and Kristen L. Kroll -- Axolotl/newt -- Malcolm Maden -- Part IV. Zebrafish -- The Zebrafish: An Overview of Its Early Development -- Nigel Holder and Qiling Xu -- Small-Scale Marker-Based Screening For Mutations in Zebrafish Development -- Peter D. Currie, Thomas F. Schilling, and Philip W. Ingham -- Transgenic Zebrafish -- Trevor Jowett -- Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg For Analysis of Gene Function -- Nigel Holder and Qiling Xu -- Retinoids in Nonmammalian Embryos -- Malcolm Maden -- Part V. Nonvertebrate Chordates -- Protochordates -- Peter W. H. Holland and Hiroshi Wada -- Part VI. Retrovirus -- Gene Transfer to the Rodent Embryo by Retroviral Vectors -- Grace K. Pavlath and Marla B. Luskin -- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses -- Cairine Logan and Philippa Francis-West -- Part VII. Molecular Techniques -- Subtractive Hybridization and Construction of cDNA Libraries -- Bruce Blumberg and Juan Carlos Izpisa͠ Belmonte -- Differential Display of Eukaryotic mRNA -- Antonio Tugores and Juan Carlos Izps̕ua Belmonte -- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers -- Anthony Graham -- Single-Cell RT-PCR cDNA Subtraction -- Damian L. Weaver, Cšar Nę͠z, Clare Brunet, Victoria Bostock, and Gerard Brady -- In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections -- Radma Mahmood and Ivor Mason. In Situ Hybridization to RNA in Whole Embryos -- Huma Shamim, Radma Mahmood, and Ivor Mason -- Wholemount In Situ Hybridization to Xenopus Embryos -- C. Michael Jones and James C. Smith -- Wholemount In Situ Hybridization to Amphioxus Embryo -- Peter W. H. Holland -- In Situ Hybridization to Sections (Nonradioactive) -- Maria Rex and Paul J. Scotting -- Immunohistochemistry Using Polyester Wax -- Andrew Kent -- Immunohistochemistry on Whole Embryos -- Ivor Mason -- Whole Embryo Assays for Programmed Cell Death -- Anthony Graham -- Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos: Optimal Ring and Roller-Bottle Culture Technique -- Jonathan Cooke and Alison Isaac -- Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting -- David I. Jackson and Clive Dickson -- Part VIII. Microscopy and Photography -- Microscopy and Photomicrography Techniques -- Richard J. T. Wingate -- Index |
Notes |
Includes index |
Bibliography |
Includes bibliographical references and index |
Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
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Methods in molecular biology (Clifton, N.J.) no:97 1064-3745 |
Subject |
Embryology -- Vertebrates -- Methodology
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Vertebrates -- Embryology -- Methodology.
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Chemical embryology -- Methodology.
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Author |
Sharpe, Paul T.
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Mason, Ivor.
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Humana Press.
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LC no. |
98023234 |
ISBN |
0896033872 alkaline paper |
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