| Description |
xiv, 378 pages : illustrations ; 24 cm |
| Series |
Methods in molecular biology |
|
Methods in molecular biology (Clifton, N.J.) ; v. 193
|
| Contents |
Machine derived contents note: Part I. Introduction -- RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA -- Joe O'Connell -- The Basics of RT-PCR: Some Practical Considerations -- Joe O'Connell -- Part II. Highly Sensitive Detection and Analysis of mRNA -- Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small Tissue Samples -- Susanne Greber-Platzer, Brigitte Balcz, Christine Fleischmann, and Gert Lubec -- Detection of mRNA Expression and Alternative Splicing in a Single Cell -- Tsutomu Kumazaki -- Nested RT-PCR: Sensitivity Controls are Essential to Determine the Biological Significance of Detected mRNA -- Triona Goode, Wen-Zhe Ho, Terry O'Connor, Sandra Busteed, Steven D. Douglas, Fergus Shanahan, and Joe O'Connell -- Part III. Quantitative RT-PCR -- Quantitative RT-PCR: A Review of Current Methodologies -- Caroline Joyce -- Rapid Development of a Quantitative-Competitive (QC) RT-PCR Assay Using a Composite Primer Approach -- Joe O'Connell, Aileen Houston, Raymond Kelly, Darren O'Brien, Aideen Ryan, Michael W. Bennett, and Kenneth Nally -- Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Products -- Franz Bachmair, Christian G. Huber, and Guenter Daxenbichler -- Time-Resolved Fluorometric Detection of Cytokine mRNAs Amplified by RT-PCR -- Kaisa Nieminen, Markus Halminen, Matti Waris, Mika Mk̃el,̃ Johannes Savolainen, Minna Sjṟoos, and Jorma Ilonen -- Mimic-Based RT-PCR Quantitation of Substance P mRNA in Human Mononuclear Phagocytes and Lymphocytes -- Jian-Ping Lai, Steven D. Douglas, and Wen-Zhe Ho -- Part IV. Detection and Analysis of RNA Viruses -- Detection and Quantification of the Hepatitis C Viral Genome -- Liam J. Fanning -- Semi-Quantitative Detection of Hepatitis C Virus RNA by "Real-Time" RT-PCR -- Joerg F. Schlaak -- RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virus -- Brian Mullan, Liam Fanning, Fergus Shanahan, and Daniel G. Sullivan -- Part V. In Situ Localization of mRNA Expression -- In Situ Immuno-PCR: A Newly Developed Method for Highly Sensitive Antigen Detection In Situ -- Yi Cao -- RT-PCR from Laser Capture Microdissected Samples -- Tatjana Crnogorac-Jurcevic, Torsten O. Nielsen, and Nick R. Lemoine -- Mycobacterium Paratuberculosis Detected by Nested PCR in Intestinal Granulomas Isolated by Laser Capture Microdissection in Cases of Crohn's Disease -- Paul Ryan, Simon Aarons, Michael W. Bennett, Gary Lee, Gerald C. O'Sullivan, Joe O'Connell, and Fergus Shanahan -- RT-PCR-Based Approaches to Generate Probes for mRNA Detection by In Situ Hybridization -- Joe O'Connell -- Part VI. Differential mRNA Expression -- Amplified RNA for Gene Array Hybridizations -- Valentina I. Shustova and Stephen J. Meltzer -- Semiquantitative Determination of Differential Gene Expression in Primary Tumors and Matched Metastases by RT-PCR: Comparison with Other Methods -- Benno Mann and Christoph Hanski -- Part VII. Genetic Analysis -- Detection of Single Nucleotide Polymorphisms Using a Non-Isotopic RNase Cleavage Assay -- Frank Waldron-Lynch, Claire Adams, Michael G. Molloy, and Fergal O'Gara -- Part VIII. RT-PCR in Immunology -- Detection of Clonally Expanded T Cells by RT-PCR-SSCP and Nucleotide Sequencing of T Cell Receptor b-CDR3 Regions -- Manae Suzuki Kurokawa, Kusuki Nishioka, and Tomohiro Kato -- Generation of scFv from a Phage Display Mini-Library Derived from Tumor-Infiltrating B Cells -- Nadg̈e Gruel, Beatrix Kotlan, Marie Beuzard, and Jean-Luc Teillaud -- Generation of Murine scFv Intrabodies from B Cell Hybridomas -- Chang Hoon Nam, Sandrine Moutel, and Jean-Luc Teillaud -- Quantitation of mRNA Levels by RT-PCR in Cells Purified by FACS: Application to Peripheral Cannabinoid Receptors in Leukocyte Subsets -- Jean Marchand and Pierre Carayon -- Part IX. RT-PCR in Antisense Technology -- Detection of Antisense RNA Transcripts by Antisense RT-PCR -- Michael C. Yeung and Allan S. Lau -- Part X. RT-PCR in cDNA Cloning -- RT-PCR in cDNA Library Construction -- Vincent Healy -- An RT-PCR-Based Protocol for the Rapid Generation of Large, Representative cDNA Libraries Suitable for Expression Screening -- Joe O'Connell -- Index |
| Summary |
Contains reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
|
Methods in molecular biology (Clifton, N.J.) no:193 1064-3745 |
| Subject |
Messenger RNA.
|
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Polymerase chain reaction -- Laboratory manuals.
|
| Genre/Form |
Laboratory manuals.
|
| Author |
O'Connell, Joe, Dr.
|
|
Humana Press.
|
| LC no. |
2002190221 |
| ISBN |
0896038750 alkaline paper |
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