| Description |
xii, 336 pages : illustrations ; 24 cm |
| Series |
Methods in molecular biology ; v. 177 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 177
|
| Contents |
Machine derived contents note: Part I. General Methods -- The Two-Hybrid System: A Personal View -- Stanley Fields and Paul L. Bartel -- Growth and Maintenance of Yeast -- Lawrence W. Bergman -- Media Formulations for Various Two-Hybrid Systems -- Michael Saghbini, Denise Hoekstra, and Jim Gautsch -- Yeast Two-Hybrid Vectors and Strains -- Philip James -- High-Efficiency Transformation of Plasmid DNA into Yeast -- Robin A. Woods and R. Daniel Gietz -- Qualitative and Quantitative Assessment of Interactions -- Monica M. Montano -- Strategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies, -- Alyson Byrd and Ren ̌St-Arnaud -- Part II. False Positives -- Two-Hybrid System and False Positives: Approaches to Detection and Elimination -- Ilya G. Serebriiskii and Erica A. Golemis -- Confirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase Pulldowns -- Dennis M. Kraichely and Paul N. MacDonald -- Two-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged Clones -- Louie Naumovski -- Part III. Applications -- Smad Interactors in Bone Morphogenetic Protein Signaling -- Xiangli Yang and Xu Cao -- Protein Interactions Important in Eukaryotic Translation Initiation -- Katsura Asano and Alan G. Hinnebusch -- Steroid Receptor and Ligand-Dependent Interaction with Coactivator Proteins -- Sergio A. Oąte -- Interaction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-Apoptosis Protein E1B-19K -- Thirugnana Subramanian and G. Chinnadurai -- Part IV. Alternative Strategies -- Mammalian Two-Hybrid Assays: Analyzing Protein-Protein Interactions in the Transforming Growth Factor- Signaling Pathway -- Xin-Hua Feng and Rik Derynck -- One-Hybrid Systems for Detecting Protein-DNA Interactions -- Mary Kate Alexander, Brenda D. Bourns, and Virginia A. Zakian -- The Split-Hybrid System: Uncoding Multiprotein Networks and Defining Mutations That Affect Protein Interactions -- Phyllis S. Goldman, Anthony J. DeMaggio, Richard H. Goodman, and Merl F. Hoekstra -- Three-Hybrid Screens: Inducible Third-Party Systems -- Bjṟn Sandrock, Franck Tirode, and Jean-Marc Egly -- Three-Hybrid Screens for RNA-Binding Proteins: Proteins Binding 3' End of Histone mRNA -- Zbigniew Dominski and William F. Marzluff -- Membrane Recruitment Systems for Analysis of Protein-Protein Interactions -- Ami Aronheim |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press |
|
Methods in molecular biology (Clifton, N.J.) no:177 1064-3745 |
| Subject |
Protein binding -- Research -- Methodology.
|
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Yeast fungi.
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Plasmids.
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| Author |
MacDonald, Paul N.
|
|
Humana Press.
|
| LC no. |
00054018 |
| ISBN |
0896038327 hardcover alkaline paper |
|
0896038084 comb alkaline paper |
|
