| Description |
xv, 373 pages : illustrations ; 24 cm |
| Contents |
Machine derived contents note: Table of contents for PCR strategies / edited by Michael A. Innis, David H. Gelfand, and John J. Sninsky. -- Bibliographic record and links to related information available from the Library of Congress catalog -- Information from electronic data provided by the publisher. May be incomplete or contain other coding. -- Principles of PCR Amplification: Preface. The Use of Co-Solvents to Enhance Amplification By the Polymerase Chain Reaction. DNA Polymerase Fidelity: Misinsertion and Mismatch Extension. Extraction of Nucleic Acids: Sample Preparation from Paraffin-Embedded Tissues. Thermostable DNA Polymerases. Direct RNA Amplification. Nucleic Acid Hybridization & Unconventional Bases. Molecular Phylogentic Analysis. The Dutp-Uracil Glycosylase System for Caryover Control and Enhanced Specificity. Quantitation. Analysis of PCR Products: Carrier Detection of Cystic Fibrosis Mutations Using PCR-Amplified DNA and a Mismatch-Binding Protein, Muts. Single-Stranded Conformational Polymorphisms (SSCP). Analysis of PCR Products By Covalent Reverse Dot Blot Hybridization. HPLC Analysis of PCR Products. Rare Mutation Detection. Heteroduplex Mobility Shift Assays for Phylogenetic Analysis. Considerations for Use of RNA: Amplified DNA Hybrid Detection Using a Monoclonal Antibody Conjugate. PCR Amplification of Vntrs. Site-Specific Mutagenesis Using the Polymerase Chain Reaction. Exact Quantification of DNA/RNA Copy Numbers By PCR/TGGE. PCR in Situ: Amplification and Detection of DNA in a Cellular Context. Chromosome Specific PCR: Maternal Blood. DNA and RNA Fingerprinting Using Arbitrarily Primed PCR. PCR-Based Screening of Yeast Artificial Chromosome (YAC) Libraries. Oligonucleotide Ligands that Discriminate Between Theophylline and Caffeine. Generation of Single Chain Antibody Fragments By PCR. Longer PCR Amplifications. Rapid Screening for Mutations Using Intercalating Dyes. Direct Analysis of Specific Bands from Arbitrarily Primed PCR Reaction. Detection of Leber's Hereditary Optic Neuropathy By Nonradioactive-LCR. Detection of Listeria Monocytogenes By PCR-Coupled Ligase Chain Reaction (LCR). 3SR as an Amplification Strategy for RNA. -- Library of Congress subject headings for this publication: Polymerase chain reaction Methodology |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Also available via the World Wide Web |
|
System requirements: Internet connectivity, World Wide Web browser, and Adobe Acrobat reader |
| Subject |
Polymerase chain reaction -- Methodology.
|
| Author |
Gelfand, David H.
|
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Innis, Michael A.
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Sninsky, John J.
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| LC no. |
94042506 |
| ISBN |
0123721822 (case : acid-free paper) |
|
0123721830 (paper : acid-free paper) |
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