Description |
xiv, 777 pages : illustrations ; 26 cm |
Contents |
1. General Methods -- 2. Bacterial Strains and Cloning Vectors -- 3. Enzymes that Modify DNA and RNA -- 4. In Vitro Amplification of DNA Using the Polymerase Chain Reaction (PCR) and the Thermostable Taq DNA Polymerase -- 5. DNA Restriction Fragment Analysis and Preparation -- 6. In Vitro Labeling of Probes and Filter Hybridization -- 7. Plasmid DNA Preparation from E coli Hosts -- 8. Preparation of DNA from Lambda Bacteriophage Clones -- 9. Subcloning Fragments into Plasmid Vectors and Plasmid Construction -- 10. Preparation of Genomic DNA -- 11. Preparation and Analysis of RNA from Eukaryotic Cells -- 12. Genomic Cloning -- 13. Preparation and Analysis of cDNA Libraries -- 14. Preparation of Subtractive cDNA -- 15. Chain Termination Sequencing -- 16. Transfection of Mammalian Cells -- 17. Basic Methods of Protein Analysis -- 18. In Situ Hybridization -- 19. Transgenic Mouse Preparation -- 20. Detection and In Vitro Generation of Specific Mutations in Genes and cDNAs -- Appendix I: Preparation of Stock Solutions -- Appendix II: Generally Useful Information -- Appendix III: Suppliers of Reagents and Equipment |
Analysis |
Molecular biology |
Bibliography |
Includes bibliographical references and index |
Subject |
Molecular biology -- Methodology.
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Molecular Biology -- methods.
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Author |
Battey, James F.
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Kuehl, W. Michael.
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LC no. |
93044281 |
ISBN |
0838506429 |
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