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Book Cover
Book
Author Davis, Leonard G.

Title Basic methods in molecular biology / Leonard G. Davis, W. Michael Kuehl, James F. Battey
Edition Second edition
Published Norwalk, Conn. : Appleton & Lange, [1994]
©1994

Copies

Location Call no. Vol. Availability
 MELB  574.88028 Dav/Bmi 1994  AVAILABLE
Description xiv, 777 pages : illustrations ; 26 cm
Contents 1. General Methods -- 2. Bacterial Strains and Cloning Vectors -- 3. Enzymes that Modify DNA and RNA -- 4. In Vitro Amplification of DNA Using the Polymerase Chain Reaction (PCR) and the Thermostable Taq DNA Polymerase -- 5. DNA Restriction Fragment Analysis and Preparation -- 6. In Vitro Labeling of Probes and Filter Hybridization -- 7. Plasmid DNA Preparation from E coli Hosts -- 8. Preparation of DNA from Lambda Bacteriophage Clones -- 9. Subcloning Fragments into Plasmid Vectors and Plasmid Construction -- 10. Preparation of Genomic DNA -- 11. Preparation and Analysis of RNA from Eukaryotic Cells -- 12. Genomic Cloning -- 13. Preparation and Analysis of cDNA Libraries -- 14. Preparation of Subtractive cDNA -- 15. Chain Termination Sequencing -- 16. Transfection of Mammalian Cells -- 17. Basic Methods of Protein Analysis -- 18. In Situ Hybridization -- 19. Transgenic Mouse Preparation -- 20. Detection and In Vitro Generation of Specific Mutations in Genes and cDNAs -- Appendix I: Preparation of Stock Solutions -- Appendix II: Generally Useful Information -- Appendix III: Suppliers of Reagents and Equipment
Analysis Molecular biology
Bibliography Includes bibliographical references and index
Subject Molecular biology -- Methodology.
Molecular Biology -- methods.
Author Battey, James F.
Kuehl, W. Michael.
LC no. 93044281
ISBN 0838506429