Title Leukemia : methods and protocols / edited by Chi Wai Eric So

Published New York : Humana Press, ©2009

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Description 1 online resource (xi, 428 pages, 6 unnumbered pages of plates) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 538 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 538 Springer protocols (Series) Contents An overview: from discovery of candidate mutations to disease modeling and transformation mechanisms of acute leukemia / C.W. So -- Backtracking of leukemic clones to birth / J. Wiemels, M. Kang and M. Greaves -- Fluorescence In situ hybridization (FISH) as a tool for the detection of significant chromosomal abnormalities in childhood leukaemia / Z.J. Konn [and others] -- Specialized fluorescence in situ hybridization (FISH) techniques for leukaemia research / L. Kearney and S. Colman -- LDI-PCR: identification of known and unknown gene fusions of the human MLL gene / C. Meyer and R. Marschalek -- Panhandle PCR approaches to cloning MLL genomic breakpoint junctions and fusion transcript sequences / B.W. Robinson and C.A. Felix -- MRD detection in acute lymphoblastic leukemia patients using Ig/TCR gene rearrangements as targets for real-time quantitative PCR / V.H. van der Velden and J.J. van Dongen -- Array-based comparative genomic hybridization as a tool for analyzing the leukemia genome / J.C. Strefford and H. Parker -- Application of SNP genotype arrays to determine somatic changes in cancer / M. Gupta and B.D. Young -- Retroviral/Lentiviral transduction and transformation assay / B.B. Zeisig and C.W. So -- Gene expression profiling of leukemia stem cells / A.V. Krivtsov [and others] -- Humanized model to study leukemic stem cells / D. Bonnet -- Model systems for examining effects of leukemia-associated oncogenes in primary human CD34+ cells via retroviral transduction / M. Wunderlich and J.C. Mulloy -- Ex vivo assays to study self-renewal and long-term expansion of genetically modified primary human acute myeloid leukemia stem cells / J.J. Schuringa and H. Schepers -- Identification and characterization of hematopoietic stem and progenitor cell populations in mouse bone marrow by flow cytometry / J. Yeung and A.C. Wai Eric So -- In vitro differentiation of mouse embryonic stem cells as a model of early hematopoietic development / P. Sroczynska [and others] -- Analysis of the mouse placenta as a hematopoietic stem cell niche / K. Ottersbach and E. Dzierzak -- Identification of protein interaction partners by the yeast two-hybrid system / M.P. Garcia-Cuellar, D. Mederer and R.K. Slany -- Complete array of HOX gene expression by RQ-PCR / G.J. Dickson, T.R. Lappin and A. Thompson -- Genome-wide determination of DNA methylation by Hpa II tiny fragment enrichment by ligation-mediated PCR (HELP) for the study of acute leukemias / M.E. Figueroa, A. Melnick and J.M. Greally -- Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins / T.A. Milne, K. Zhao and J.L. Hess Summary Recent advances in molecular and cellular biology techniques have significantly improved our ability to detect, monitor, model and study the underlying molecular basis and pathogenesis of leukemia, yet we are still in an early discovery stage and much more work is needed in order to develop better strategies to diagnose, classify and treat this biologically and clinically diverse disease. In Leukemia: Methods and Protocols, expert researchers bring together a wide range of state-of-the-art laboratory methods and detailed protocols that are useful for both clinical and basic research scientists working on the disease. The volume provides techniques for prenatal backtracking of leukemic clone, molecular diagnosis, detection of genome-wide genetic abnormalities and profiling, identification of unknown fusion genes, monitoring of minimal residual diseases, disease modeling using murine and human primary hematopoietic cells, studying of normal and malignant hematopoiesis, identification of interacting partners with leukemia associated oncoproteins, and global characterization of genome-wide epigenetic changes in leukemic cells. Written in the highly successful Methods in Molecular Biology series format, the convenient chapters contain brief introductions, lists of the necessary materials, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Leukemia: Methods and Protocols will help researchers to advance knowledge and have a better understanding of the disease, which will ultimately facilitate development of anti-cancer therapy and improve quality of life for patients Analysis leukemie leukaemia medisch onderzoek medical research diagnostische technieken diagnostic techniques laboratoriummethoden laboratory methods protocollen protocols Medicine (General) Geneeskunde (algemeen) Bibliography Includes bibliographical references and index Notes English Print version record Subject Acute leukemia -- Molecular aspects Acute leukemia -- Pathogenesis Acute leukemia -- Molecular aspects -- Research -- Methodology Leukemia. Leukemia Leukemia Leukämie Molekularbiologie Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author So, Chi Wai Eric ISBN 9781597454186 1597454184

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