Title Methods in mouse atherosclerosis / edited by Vicente Andrés, Beatriz Dorado

Published New York : Humana Press : Springer, ©2015

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Description 1 online resource (xiv, 400 pages) : illustrations (some color) Series Methods in molecular medicine ; 1339 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 1339, 1064-3745 Springer protocols (Series) 1949-2448 Contents Use of mouse models in atherosclerosis research / Godfrey S. Getz and Catherine A. Reardon -- Cytokines and immune responses in murine atherosclerosis / Pascal J.H. Kusters and Esther Lutgens -- Intravital microscopy for atherosclerosis research / Remco T.A. Megens and Oliver Soehnlein -- Noninvasive molecular imaging of mouse atherosclerosis / Martina A. McAteer and Robin P. Choudhury -- Oil red O and hematoxylin and eosin staining for quantification of atherosclerosis burden in mouse aorta and aortic root / M. Jesús Andrés-Manzano, Vicente Andrés, and Beatriz Dorado -- Isolation, culture, and polarization of murine bone marrow-derived and peritoneal macrophages / Inés Pineda-Torra [and others] -- Isolation of mouse primary aortic endothelial cells by selection with specific antibodies / Pedro Molina-Sánchez and Vicente Andrés -- Isolation and culture of aortic smooth muscle cells and in vitro calcification assay / Ricardo Villa-Bellosta and Magda R. Hamczyk -- Immunostaining of macrophages, endothelial cells, and smooth muscle cells in the atherosclerotic mouse aorta / Prashanthi Menon and Edward A. Fisher -- Immunostaining of lymphocytes in mouse atherosclerotic plaque / Anton Gisterå and Daniel F.J. Ketelhuth -- Flow cytometric analysis of immune cells within murine aorta / Breanne N. Gjurich, Parésa L. Taghavie-Moghadam, and Elena V. Galkina -- In vitro differentiation of naïve CD4+ T cells : a tool for understanding the development of atherosclerosis / Salvador Iborra and Jose M. González-Granado -- Quantification of apoptosis in mouse atherosclerotic lesions / Nichola L. Figg and Martin R. Bennett -- Quantification of cellular proliferation in mouse atherosclerotic lesions / José J. Fuster -- Quantification of in vitro macrophage cholesterol efflux and in vivo macrophage-specific reverse cholesterol transport / Joan Carles Escolà-Gil [and others] -- In vitro macrophage phagocytosis assay / Magda R. Hamczyk, Ricardo Villa-Bellosta, and Vicente Andrés -- Glucose and insulin tolerance tests in the mouse / Ángela Vinué and Herminia González-Navarro -- Wire myography to study vascular tone and vascular structure of isolated mouse arteries / Lara del Campo and Mercedes Ferrer -- Pressure myography to study the function and structure of isolated small arteries / Olav L. Schjørring, Rune Carlsson, and Ulf Simonsen -- Generation of aorta transcript atlases of wild-type and apolipoprotein E-null mice by laser capture microdissection-rased mRNA expression microarrays / Changjun Yin [and others] -- Analysis of gene and protein expression in atherosclerotic mouse aorta by western blot and quantitative real-time PCR / José Rivera-Torres -- Bone marrow transplantation in mice to study the role of hematopoietic cells in atherosclerosis / Vinatha Sreeramkumar and Andrés Hidalgo -- Tandem stenosis to induce atherosclerotic plaque instability in the mouse / Yung Chih Chen, Jennifer Rivera, and Karlheinz Peter -- Detection of intraplaque hemorrhage in mouse atherosclerotic lesions / Judith C. Sluimer, Marion J. Gijbels, and Sylvia Heeneman -- Mechanical stabilization of mouse carotid artery for in vivo intravital microscopy imaging of atherogenesis / Raphaël Chèvre -- Intravital microscopy in the cremaster muscle microcirculation for endothelial dysfunction studies / Cristina Rius and María J. Sanz -- Fluorescent molecular tomography for in vivo imaging of mouse atherosclerosis / Alicia Arranz [and others] -- In vivo 18F-FDG-PET imaging in mouse atherosclerosis / Jesús Mateo [and others] -- Magnetic resonance imaging of the atherosclerotic mouse aorta / Jesús Mateo [and others] Summary This volume reviews available mouse models of atherosclerosis, basic aspects of disease initiation and progression, and the visualization of atherosclerotic lesions with invasive and non-invasive techniques that are widely used both in basic research and in the clinic. The chapters catalogue a wide range of in vitro and in vivo experimental methods used for atherosclerosis research, including thorough protocols and valuable notes based on the authors' personal wet lab experience Bibliography Includes bibliographical references and index Subject Medicine. Molecular biology. Cardiology. Animal models in research. Atherosclerosis -- Animal models Mice as laboratory animals. Mice. Animals Mice Atherosclerosis Models, Animal Medicine Molecular Biology Cardiology medicines (material) molecular biology. medicine (discipline) Cardiovascular medicine. Animal pathology & diseases. Medical research. Medical -- Cardiology. Science -- Life Sciences -- Zoology -- General. Science -- Life Sciences -- Biology -- Molecular Biology. Mice Animal models in research Atherosclerosis -- Animal models Cardiology Medicine Mice as laboratory animals Molecular biology Genre/Form Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Dorado, Beatriz. Andrés, Vicente. ISBN 9781493929290 1493929291 1493929283 9781493929283

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