| Description |
1 online resource (xxxiv, 284 pages) : illustrations |
| Series |
Methods in enzymology, 0076-6879 ; v. 421 |
|
Methods in enzymology ; v. 421. 0076-6879
|
| Contents |
Strain collections and genetic nomenclature / Stanley R. Maloy and Kelly T. Hughes -- Use of antibiotic-resistant transposons for mutagenesis / Stanley R. Maloy -- In vivo mutagenesis using EZ-Tn5 / John R. Kirby -- Identification of essential genes in bacteria / David R. Hillyard and Michael J. Reed -- Isolation of transposon insertions / Stanley R. Maloy -- Localized mutagenesis / Stanley R. Maloy -- Generation of deletions and duplications using transposons as portable regions of homology with emphasis on Mud and Tn10 transposons / Kelly T. Hughes -- Target-directed proteolysis in vivo / Markus Eser ... [et al.] -- Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering / Beth Traxler and Eliora Gachelet -- Using genomic microarrays to study insertional/transposon mutant libraries / David N. Baldwin and Nina R. Salama -- Screening transposon mutant libraries using full-genome oligonucleotide microarrays / Kelly M. Winterberg and William S. Reznikoff -- Creating recombination-activated genes and sequence-defined mutant libraries using transposons / Larry Gallagher ... [et al.] -- Use of operon and gene fusions to study gene regulation in Salmonella / Kelly T. Hughes and Stanley R. Maloy -- Genomic screening for regulatory genes using the T-POP transposon / Changhan Lee ... [et al.] -- Recombineering: In vivo genetic engineering in E. coli, S. enterica, and beyond / James A. Sawitzke ... [et al.] -- [lambda]-red genetic engineering in Salmonella enterica serovar Typhimurium / Joyce E. Karlinsey -- Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements / Nathalie Garcia-Russell, Samantha S. Orchard, and Anca M. Segall -- Dissecting nucleic acid-protein interactions using challenge phage / Stanley R. Maloy and Jeffrey Gardner -- Mud-P22 / Jenny A. Crain and Stanley R. Maloy -- Phage metagenomics / Veronica Casas and Forest Rowher |
| Summary |
A volume of indispensable methods for the use of transposons and phage in genomic engineering |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Description based on print version record |
| Subject |
Bacterial genetics.
|
|
Bacteriophages.
|
|
Transposons.
|
|
Bacteria -- genetics.
|
|
DNA, Bacterial.
|
| Form |
Electronic book
|
| Author |
Hughes, Kelly Thomas.
|
|
Maloy, Stanley R.
|
| ISBN |
0080475108 (electronic bk.) |
|
0123737494 (electronic bk.) |
|
9780080475103 (electronic bk.) |
|
9780123737496 (electronic bk.) |
|
