| Description |
1 online resource (x, 209 pages) : illustrations (some color) |
| Series |
Springer protocols |
|
Methods in molecular biology, 1940-6029 ; 706 |
|
Springer protocols (Series)
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Methods in molecular biology (Clifton, N.J.) ; v. 706
|
| Contents |
Cell-based microarrays: Overview / E. Palmer -- Cell-based co-transfection microarrays for use with hek293t cells on a poly d-lysine-coated polystyrene microplate / M. Soni and F. Lai -- Large-scale cell-based microarrays and their use with hek293t cells and downstream apoptotic assays / E. Palmer and T.C. Freeman -- A novel fluorescent transcriptional reporter for cell-based microarray assays / T.M. Redmond and M.D. Uhler -- High-throughput subcellular protein localization using transfected-cell arrays. Subcellular protein localization using cell arrays / Y. Hu and M. Janitz -- Cell arrays for the measurement of organelle dynamics in living cells / H. Erfle [and others] -- High-throughput immunofluorescence microscopy using yeast spheroplast cell-based microarrays / W. Niu, G.T. Hart and E.M. Marcotte -- Cell-based microarrays of infectious adenovirus encoding short hairpin rna (shrna) / H. Volkmer and F. Weise -- Reverse transfected cell microarrays in infectious disease research / A. Konrad [and others] -- Transfected cell microarrays for the expression of membrane-displayed single-chain antibodies / B. Lin and J.B. Delehanty -- Blood cell capture on antibody microarrays and monitoring of the cell capture using surface plasmon resonance imaging / Y. Roupioz [and others] -- Immobilized culture and transfection microarray of non-adherent cells / S. Yamaguchi, E. Matsunuma and T. Nagamune -- Plasma polymer and peg-based coatings for DNA, protein and cell microarrays / A.L. Hook, N.H. Voelcker and H. Thissen -- Polymer microarrays for cellular high-content screening / S. Pernagallo and J.J. Diaz-Mochon -- High-throughput analyses of gene functions on a cell chip by electroporation / K. Kato and H. Iwata -- Microfluidic image cytometry / K. Kamei [and others] |
| Summary |
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins |
| Analysis |
celbiologie |
|
cellular biology |
|
levenswetenschappen |
|
life sciences |
|
Biology (General) |
|
Biologie (algemeen) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Biochips.
|
|
Pharmaceutical technology.
|
|
Microchip Analytical Procedures
|
|
Technology, Pharmaceutical
|
|
Microarray Analysis
|
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High-Throughput Screening Assays
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Chemistry Techniques, Analytical
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Investigative Techniques
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Analytical, Diagnostic and Therapeutic Techniques and Equipment
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Pharmaceutical technology
|
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Biochips
|
| Genre/Form |
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Palmer, Ella.
|
| ISBN |
9781617379703 |
|
1617379700 |
|
