| Description |
1 online resource (xi, 524 pages) : illustrations (some color) |
| Series |
Springer protocols handbooks, 1949-2448 |
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Springer protocols (Series), 1949-2448
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| Contents |
Intro -- Preface -- Contents -- Contributors -- Part I: Orchid Seeds in Plant Propagation and Conservation -- Chapter 1: Understanding Seed and Protocorm Development in Orchids -- 1 Introduction -- 2 The Orchid Seeds -- 2.1 The Embryo -- 2.1.1 Suspensor -- 2.1.2 Embryo Proper -- 2.2 Endosperm -- 2.3 The Seed Coat -- 2.4 Phytohormone Studies -- 3 Seed Germination -- 3.1 Asymbiotic Seed Germination of Terrestrial Orchids -- 3.2 Asymbiotic Seed Germination of Epiphytic Orchids -- 3.3 Changes in Protocorms Associated with Asymbiotic Seed Germination |
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3.4 Symbiotic Seed Germination: Changes in Protocorms Associated with Symbiotic Seed Germination -- 3.5 Protocorm and the Shoot Apical Meristem -- 4 Future Perspectives -- References -- Chapter 2: Orchid Mycorrhizal Fungi: Isolation and Identification Techniques -- 1 Introduction -- 2 Discussion of Technical Issues and Concerns -- 2.1 Terminology -- 2.2 Spotting Small Seedlings in the Field -- 2.3 Identifying Small Seedlings to Taxonomic Rank -- 2.4 Avoiding Senile Populations -- 2.5 Permits and Transport -- 3 Fungal Isolation -- 3.1 Field Collection -- 3.2 Peloton Assessment |
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3.3 Surface Sterilization -- 3.4 Fungal Isolation from Root Pieces -- 3.5 Fungal Isolation from Pelotons in Cortical Cells and Protocorms -- 3.6 Subculturing and Storage -- 3.7 Summary -- 4 Fungal Identification -- 4.1 Background -- 4.2 Modern Approaches to Identification -- 4.3 Geographical Patterns of Rhizoctonia-Like Fungi -- 4.4 Provisional Identification of Rhizoctonia-Like Fungi: A Practical First Step -- 4.5 Cultural Characteristics of the Cantharellales and Sebacinales -- 4.5.1 Order Cantharellales -- 4.5.2 Order Sebacinales -- 4.6 Molecular Identification of Orchid Mycorrhizal Fungi |
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Chapter 4: Orchid Seed and Pollen: A Toolkit for Long-Term Storage, Viability Assessment and Conservation -- 1 Introduction -- 2 Species Identification -- 2.1 Procedure for Species Identification -- 3 Pollination for Seed Production -- 3.1 Procedure for Pollinating Plants -- 4 Pollen Storage -- 4.1 Procedure for Pollen Storage -- 4.2 Pollen Germination -- 5 Pollen and Seed Exchange -- 5.1 Guidelines for Pollen and Seed Exchange -- 5.2 Procedure for Sending Seed and Pollen Through the Post -- 6 Harvesting Seed -- 6.1 Procedure for Seed Collection and Handling -- 6.2 Ex Situ Collections |
| Summary |
The orchid family is one of the largest families of flowering plants known for their beauty and economic importance. This work provides information in key areas of research that are important to both scientists and commercial growers alike. The main purposes of this book are to provide key practical areas of research, such as, germination, micropropagation, traditional and current techniques related to plant improvement; document methods that ensure survival of plants from laboratories to greenhouses; promote communication between scientists and growers, so that their combined expertise on these areas will lead to the successful growth of orchids in their natural habitats or commercial greenhouses. This book can serve as reference for laymen with an interest in orchid growing. This book is divided into 5 parts. The first part emphasizes propagation methods using seeds and related techniques that are important to plant conservation and improvement. Successes in asymbiotic and symbiotic seed germination are keys to orchid conservation and their propagation. The second part summarizes micropropagation methods, common media, and newer methods of micropropagation such as the bioreactor culture procedures. The third part focuses on techniques related to the manipulation of explants in an in vitro environment. The fourth part covers cell biological methods and transformation techniques. Since the successes in a laboratory setting do not guarantee plant survival and propagation in greenhouses and in the natural environment, it discusses greenhouse propagation techniques that are essential to the survival of plants generated from a laboratory setting. The fifth part showcases recent successes on orchid propagation by documenting sample publications and how to present orchids in an artistic fashion for one's enjoyment |
| Notes |
Includes index |
| Bibliography |
References-Chapter 3: Protocols for Establishing Fungi-Protocorm Culture-1 Introduction-2 Materials-2.1 Laboratory Equipment and Supplies-2.2 Media and Chemicals-2.3 Preparation of the Seed Baiting Packet-2.4 Equipment and Materials Associated with Molecular Biology Studies-3 Methods-3.1 Orchid Seeds Baiting In Situ-3.2 Fungal Isolation-3.3 In Vitro Symbiotic Seed Germination-3.4 Identification of Germination-ƯPromoting Fungus-4 Concluding Remarks-5 Notes-References |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed May 14, 2018) |
| Subject |
Orchids -- Breeding -- Laboratory manuals
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Orchids -- Biotechnology -- Laboratory manuals
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Orchids -- Biotechnology
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Orchids -- Breeding
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Lee, Y.-I. (Yung-I.), editor.
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Yeung, Edward C., editor.
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| ISBN |
9781493977710 |
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1493977717 |
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