| Description |
1 online resource (xvii, 415 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; v. 173 |
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Methods in molecular biology (Clifton, N.J.) ; . v. 173.
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| Contents |
Part III. Methods and techniques to study calcium-binding proteins ; Quantitative analysis of Ca²-binding by flow dialysis / Michio Yazawa -- Calcium binding to proteins studied via competition with chromophoric chelators / Sara Linse -- Deconvolution of calcium-binding curves : facts and fantasies / Jacques Haiech and Marie-Claude Kilhoffer -- Absorption and circular dichroism spectroscopy / Stephen R. Martin and Peter M. Bayley -- Fourier transform infrared spectroscopy of calcium-binding proteins / Heinz Fabian and Hans J. Vogel -- Steady-state fluorescence spectroscopy / Aalim M. Weljie and Hans J. Vogel -- Fluorescence methods for measuring calcium affinity and calcium exchange with proteins / J. David Johnson and Svetlana B. Tikunova -- Surface plasmon resonance of calcium-binding proteins / Karin Julenius -- Differential scanning calorimetry / Maria M. Lopez and George I. Makhatadze -- Isothermal titration calorimetry / Maria M. Lopez and George I. Makhatadze -- Multiangle laser light scattering and sedimentation equilibrium / Leslie D. Hicks, Jean-René Alattia, Mitsuhiko Ikura, and Cyril M. Kay -- Small-angle solution scattering reveals information on conformational dynamics in calcium-binding proteins and in their interactions with regulatory targets / Jill Trewhella and Joanna K. Krueger -- Investigation of calcium-binding proteins using electrospray ionization mass spectrometry / Amanda L. Doherty-Kirby and Gilles A. Lajoie -- Synthetic calcium-binding peptides / Gary S. Shaw -- Proteolytic fragments of calcium-binding proteins / Richard D. Brokx and Hans J. Vogel |
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Electron magnetic resonance studies of calcium-binding proteins / Lawrence J. Berliner -- Cadmium-113 and lead-207 NMR spectroscopic studies of calcium-binding proteins / Teresa E. Clarke and Hans J. Vogel -- Calcium-43 of NMR of calcium-binding proteins / Torbj̲örn Drakenberg -- Exploring familial relationships using multiple sequence alignment / Aalim M. Weljie and Jaap Heringa -- Structure determination by NMR : isotope labeling / Monica X. Li, David C. Corson, and Brian D. Sykes -- Protein structure calculation from NMR data / Tapas K. Mal, Stefan Bagby, and Mitsuhiko Ikura -- Shape and dynamics of a calcium-binding protein investigated by nitrogen-15 NMR relaxation / Jörn M. Werner, Iain D. Campbell, and A. Kristina Downing -- The use of dipolar couplings for the structure refinement of a pair of calcium-binding EGF domains / Jonathan Boyd, Iain D. Campbell, and A. Kristina Downing -- Vector geometry mapping : a method to characterize the conformation of helix-loop-helix calcium-binding proteins / Kyoko L. Yap, James B. Ames, Mark B. Swindells, and Mitsuhiko Ikura -- Use of calmodulin antagonists and S-100 protein interacting drugs for affinity chromatography / Ryoji Kobayashi -- Enzymatic assays to compare calmodulin isoforms, mutants, and chimeras / Michael P. Walsh, Jacquelyn E. Van Lierop, Cindy Sutherland, Ritsu Kondo, and J. David Johnson -- Gene expression in transfected cells / Kate Hughes, Juha Saarikettu, and Thomas Grundström -- Monitoring the intracellular free Ca²-calmodulin concentration with genetically-encoded fluorescent indicator proteins / Anthony Persechini -- Studying the spatial distribution of Ca²⁺-binding proteins : how does it work for calmodulin? / Katalin Török, Richard Thorogate, and Steven Howell |
| Summary |
Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols- Volume 1: Reviews and Case Histories and Volume 2: Methods and Techniques, Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant class proteins, and provide a comprehensive collection of proven experimental techniques for studying it both in vitro and in vivo. This second volume focuses on cutting-edge experimental techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding protein in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells. The first companion volume, Reviews and Case Histories sets the stage for this volume by introducing the various classes of intra- and extracellular calcium-binding proteins and their mode of action. Comprehensive and highly practical, the two volumes of Calcium-Binding Protocols provide experimental and clinical biologists with a host of advanced experimental methods that can be applied successfully to the study of both existing and newly discovered members of this critically important class of proteins |
| Bibliography |
Includes bibliographical references at chapter ends, and index |
| Notes |
Print version record |
| Subject |
Calcium-binding proteins -- Research -- Methodology
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Biochemistry.
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Life sciences.
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Calcium-binding proteins.
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Calcium-Binding Proteins
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Biochemistry
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Biological Science Disciplines
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Research Design
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biochemistry.
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biological sciences.
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SCIENCE -- Life Sciences -- Biochemistry.
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Calcium-binding proteins
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Biochemistry
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Life sciences
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Vogel, Hans J., editor
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| ISBN |
9781592591848 |
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0585419728 |
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9780585419725 |
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1592591841 |
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9786610821464 |
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6610821461 |
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1280821469 |
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9781280821462 |
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