| Description |
1 online resource (xiii, 359 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 114 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 114
|
| Contents |
Part I. General -- 1. Fluorescent Measurement of [Ca2+]c : Basic Practical Considerations / Alec W.M. Simpson -- 2. Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 / Robert A. Hirst [and others] -- 3. Measurement of [Ca2+]i in Cell Suspensions Using lndo-1 / Adriaan Nelemans -- Part II. Specialist Measurement Systems -- 4. Confocal Microscopy : Theory and Applications / Laura C. Mongan [and others] -- 5. Measurement of Intracellular Calcium Concentration Using Confocal Microscopy / Carmen Perez-Terzic, Marisa Jaconi, and Lisa Stehno-Bittel -- 6. Single Cell and Subcellular Measurement of Intracellular Ca2+ Concentration ([Ca2+]i) / Anthony J. Morgan and Andrew P. Thomas -- 7. Measurement of [Ca2+] Using the Fluorometric Imaging Plate Reader (FLIPR) / Elaine Sullivan, Emily M. Tucker, and Ian L. Dale -- Part III. Nonelectrophysiological Measurement of Ca2+ Channel Activity -- 8. Measurement of Ca2+ Entry Using 45Ca2+ / Mercedes Villaroya [and others] -- 9. Measurement of [3H]PN200-110 and [125I]w-Conotoxin MVIIA Binding / Kazuyoshi Hirota and David G. Lambert -- Part IV. Measurement of INS(1,4,5)P3 and Ca2+ Release from Intracellular Stores -- 10. Measurement of Inositol (Poly)Phosphate Formation Using [3H]Inositol Labeling Protocols in Permeabilized Cells / Philip Swigart and Shamshad Cockcroft -- 11. Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay / Darren Smart -- 12. Measurement of Calcium Fluxes in Permeabilized Cells Using a45Ca2+ Uptake and Release Assay / Robert A. Wilcox -- 13. Microinjection of Myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents into Intact Adherent Cells / Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann -- 14. Photolysis of Caged Calcium Using a Low-Cost Flash Unit / Gommert A. van Koeveringe and Ron van Mastrigt -- 15. Measurement of Ca2+ Flux through Ins(1,4,5)P3 Receptor-Ca2+ Channels in Lipid Bilayers ("Dip-Tip" and "Schindler" Methodology) / Edwin C. Thrower -- 16. Continuous Fluorescent Monitoring of Cellular Calcium Fluxes: A Novel Perfusion System for the Investigation of Inositol (1,4,5)Triphosphate-Dependent Quantal Calcium Release Using Immobilized, Electropermealized Cells / Robert A. Wilcox and James Strupish -- 17. Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators / Aldebaran M. Hofer -- Part V. Specialized Measurement Techniques -- 18. Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry / Hideo Kanaide -- 19. Measurement of Calcium and Movement in Heart Cells / Leong L. Ng and Paulene A. Quinn -- 20. Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations / Paul Martinez-Zaguilan [and others] -- 21. Measurement of Cytosolic-Free Ca2+ in Plant Tissue / Martin R. McAinsh and Irina Staxen -- Part VI. Ca2+ Sensitive Targets -- 22. Assay and Purification of Calmodulin-Dependent Protein Kinase / Rakesh Kakkar and Rajendra K. Sharma -- 23. Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka |
| Summary |
In Calcium Signaling Protocols, David Lambert and a panel of leading authorities present a wide range of experimental protocols for studying Ca2+ signaling. These optimized techniques cover the more common applications, including 45Ca2+ flux measurements, and basic fluorometric technology, as well as more sophisticated methods, including confocal microscopy and subcellular Ca2+ imaging. There are also methods-largely based on fluorescence measurement-to determine Ca2+ channel activity and the release of Ca2+ from intracellular stores. In addition, there are methods to assess Ca2+ -sensitive target site activity. Calcium Signaling Protocols offers today's researchers readily reproducible laboratory methods that make it possible to examine the calcium signaling process in detail in a range of cells of animal and plant origin. These cutting-edge techniques will be of enormous value to all those working to understand not only cell signaling, but also the mode of action of a range of pharmacological agents |
| Analysis |
signalen |
|
signals |
|
signaaltransductie |
|
signal transduction |
|
calciumionen |
|
calcium ions |
|
Biochemistry |
|
Biochemie |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Calcium -- Laboratory manuals
|
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Calcium -- Research -- Methodology
|
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Calcium channels -- Research -- Methodology
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Cellular signal transduction -- Research -- Methodology
|
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Cellular signal transduction.
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Calcium -- pharmacokinetics
|
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Calcium Channels -- pharmacokinetics
|
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Calcium Signaling -- physiology
|
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Signal Transduction
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Spectrometry, Fluorescence -- methods
|
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SCIENCE -- Life Sciences -- Biochemistry.
|
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Cellular signal transduction
|
|
Calcium
|
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Cellular signal transduction -- Research -- Methodology
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Lambert, David G
|
| ISBN |
9781592592500 |
|
1592592503 |
|
0585295425 |
|
9780585295428 |
|
