| Description |
1 online resource (509 pages) : illustrations (some color) |
| Series |
Methods in signal transduction |
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Methods in signal transduction.
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| Contents |
Front cover; Series Preface; Preface to the Second Edition; Editor; Contributors; Table of Contents; 1 Fluorescent Calcium Indicators Based on BAPTA; CONTENTS; 1.1 INTRODUCTION; 1.2 CALCIUM INDICATORS BASED ON BAPTA: GENERAL CONSIDERATIONS; 1.3 HIGH-AFFINITY CALCIUM INDICATORS; 1.3.1 HIGH-AFFINITY RATIOMETRIC INDICATORS; 1.3.1.1 High-Affinity Ratiometric Indicators Excited by Ultraviolet Light (Fura-2, Benzothiaza-1, Benzothiaza-2, and Indo-1); 1.3.1.2 High-Affinity Ratiometric Indicators Excited by Visible Light (Fura Red); 1.3.1.3 Summary of the Ratiometric Indicators |
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1.3.2 HIGH-AFFINITY NONRATIOMETRIC CALCIUM INDICATORS1.3.2.1 Indicators Based on Fluorescein Derivatives (Fluo-3, Fluo-4, Calcium Green-1, Calcium Green-2, Oregon Green 488 BAPTA); 1.3.2.2 Indicators Based on Rhodamine (Rhod-2, X-Rhod-2, Calcium Orange, Calcium Crimson); 1.3.2.3 Summary of Nonratiometric Indicators; 1.4 LOW-AFFINITY CALCIUM INDICATORS FOR MEASURING LARGE OR RAPID CALCIUM TRANSIENTS; 1.4.1 THE RELATIONSHIP BETWEEN AFFINITY AND KINETICS; 1.4.2 DESIGN OF LOW-AFFINITY INDICATORS; 1.4.3 LOW-AFFINITY CALCIUM INDICATORS BASED ON APTRA |
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1.4.4 TETRACARBOXYLATE LOW-AFFINITY CALCIUM INDICATORS1.4.4.1 The 5-Nitro BAPTAs (Fluo-5N, Calcium Green-5N, Calcium Orange-5N, Oregon Green 488 BAPTA-5N); 1.4.4.2 Benzothiazole Derivatives (Benzothiaza-1 and -2, BTC); 1.4.4.3 The Halogenated BAPTA Moderate- to Low-Affinity Indicators (BAPTA-4F, -5F, -5Cl, -6F, and -FF Derivatives); 1.4.5 SUMMARY OF THE LOW-AFFINITY CALCIUM INDICATORS; 1.5 INDICATORS THAT RESIST LEAKAGE AND SEQUESTRATION; 1.5.1 INDICATOR-DEXTRAN CONJUGATES; 1.5.2 FURA-PE3 AND RELATED INDICATORS; 1.5.3 SUMMARY OF THE LEAKAGE-RESISTANT CALCIUM INDICATORS |
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1.6 NEAR-MEMBRANE CALCIUM INDICATORS1.6.1 THE C18 NEAR-MEMBRANE CALCIUM INDICATORS (C18-FURA-2, CALCIUM GREEN C18); 1.6.1.1 C18-Fura-2 (Fura-2-C18); 1.6.1.2 Calcium Green C18; 1.6.1.3 Fura-FFP18 and Related Calcium Indicators; 1.6.2 SUMMARY OF THE NEAR-MEMBRANE CALCIUM INDICATORS; 1.7 USE OF CALCIUM INDICATORS WITH MULTIPHOTON MICROSCOPY; 1.8 CONCLUSION; ACKNOWLEDGMENTS; REFERENCES; 2 Fluorescent Indicators -Facts and Artifacts; CONTENTS; 2.1 INTRODUCTION; 2.1.1 SIGNALINGCA; 2.2 ANATOMY OF A CALCIUM SIGNAL; 2.2.1 SIGNALMONITORING; 2.2.1.1 Fluorescent Indicator Selection; 2.2.1.2 Fura-2 |
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2.2.1.3 Fura-5FTABLE 2.1 General Characteristics of Fluorescent Calcium Indicator in General Use; TABLE 2.2 Spectral and Biological Characteristics of Ca; 2.2.1.4 Fluorescent Indicators and Cytoplasmic Loading; 2.2.1.5 Quantitative Assessment of a [Ca; 2.2.2 SIGNALDISSECTING; 2.2.2.1 Resting [Ca] and the ''Calcium Re-Addition'' Protocol; 2.2.2.2 Receptor-Activated Cells and Biphasic Calcium Signaling; 2.2.3 CAINTRACELLULARCA; 2.2.3.1 Manipulating Intracellular Ca; 2.2.3.2 Protocol for Defining Intracellular Ca; 2.3 Ca ENTRY; 2.3.1 MONITORING CA; 2.3.1.1; 2.3.1.2; 2.3.1.3 |
| Summary |
Fluorescent Calcium Indicators Based on BAPTA; Martin Poenie Fluorescent Indicators-Facts and Artifacts; Gary St. J. Bird and James W. Putney Jr. Fluorescence Microplate-Based Techniques for the High-Throughput Assessment of Calcium Signaling: The Highs and Lows for Calcium Researchers; Gregory R. Monteith and Gary St. J. Bird Genetically Encoded Fluorescent Calcium Indicator Proteins; Atsushi Miyawaki, Takeharu Nagai, and Hideaki Mizuno Targeted Aequorins; Andrea Prandini and Rosario Rizutto Electrophysiological Recordings of Ca2 Currents; Anant B. Parekh Molecular Biology of Ca2 Chann |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Calcium -- Physiological effect.
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Calcium channels.
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Cellular signal transduction.
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Calcium -- physiology
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Calcium Channels
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Signal Transduction
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Calcium Channels -- physiology
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Signal Transduction -- physiology
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SCIENCE -- Life Sciences -- Biochemistry.
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Calcium channels
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Calcium -- Physiological effect
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Cellular signal transduction
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| Form |
Electronic book
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| Author |
Putney, James W
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| ISBN |
1420038230 |
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9781420038231 |
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1280639636 |
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9781280639630 |
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9786610639632 |
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6610639639 |
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9780429124099 |
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0429124090 |
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0849327830 |
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9780849327834 |
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