Title Calcium signaling protocols

Edition 3rd ed. 2013 Published Totowa, NJ : Humana Press : Imprint: Humana Press, 2013

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Description 1 online resource (XI, 360 pages 51 illustrations, 17 illustrations in color.) Series Methods in molecular biology Calcium signaling protocols Methods in molecular biology (Clifton, N.J.) ; v. 937. 1064-3745 Contents Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations -- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 -- Confocal Microscopy: Theory and Applications for Cellular Signaling -- Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer -- Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader (FLIPR®) -- Ratiometric [Ca2+]i Measurements in Adherent Cell-lines Using the NOVOstar Microplate Reader -- Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents in Single Cells: Heterologous Expression Systems and Neurones -- Combined Calcium Fluorescence Recording with Ionic Currents in Contractile Cells -- Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol Labeling Protocols in Permeabilized Cells -- Single Cell Imaging Techniques for the Real-Time Detection of IP3 in Live Cells -- Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay -- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry -- Calcium Measurements from Whole Heart Using Rhod-2.- Measurement of Changes in Endothelial and Smooth Muscle Ca2+ in Pressurized Arteries -- Single Cell and Subcellular Measurements of Intracellular Ca2+ Concentration -- Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations -- Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes -- Chimeric G-Proteins in Fluorimetric Calcium Assays: Experience with Opioid Receptors -- Compartmentalizing Genetically-Encoded Calcium Sensors -- Measurement of Cytosolic-Free Ca2+ in Plant Tissue -- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1) Summary The regulation of intracellular Ca2+ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued. With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca2+ regulation and provided much new information on processes. Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca2+ signalling for screening, and genetically encoded sensors. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca2+ Analysis calcium biochemie biochemistry Biochemistry Biochemie Bibliography Includes bibliographical references and index Notes English Subject Calcium -- Research -- Methodology -- Laboratory manuals Calcium -- Research -- Methodology Calcium channels -- Research -- Methodology Cellular signal transduction. Blood coagulation factors. Alkaline earth metals. Ion channels. Cell physiology. Life sciences. Chemical elements. Physical sciences. Metals. Glycoproteins. Biological transport. Photometry. Inorganic compounds. Carrier proteins. Membrane proteins. Metabolism. Proteins. Calcium channels. Calcium. Pharmacokinetics. Physiology. Fluorimetry. Biological products. Calcium -- pharmacokinetics Calcium Channels -- pharmacokinetics Calcium Signaling -- physiology Fluorometry -- methods Signal Transduction Blood Coagulation Factors Ion Transport Luminescent Measurements Metals, Alkaline Earth Second Messenger Systems Ion Channels Biochemical Phenomena Kinetics Cell Physiological Phenomena Publication Formats Biological Science Disciplines Elements Natural Science Disciplines Metals Membrane Glycoproteins Membrane Transport Proteins Publication Characteristics Biological Factors Biological Transport Chemical Phenomena Photometry Inorganic Chemicals Chemicals and Drugs Disciplines and Occupations Carrier Proteins Chemistry Techniques, Analytical Membrane Proteins Metabolism Phenomena and Processes Proteins Investigative Techniques Amino Acids, Peptides, and Proteins Analytical, Diagnostic and Therapeutic Techniques and Equipment Calcium Signaling Calcium Channels Laboratory Manuals Calcium Pharmacokinetics Physiology Fluorometry biological sciences. physical sciences. metal. metabolism (biological concept) protein. calcium. physiology. elements (chemical substances) Proteins Physiology Physical sciences Photometry Pharmacokinetics Metals Metabolism Membrane proteins Life sciences Ion channels Inorganic compounds Glycoproteins Fluorimetry Chemical elements Cell physiology Carrier proteins Calcium channels Calcium Blood coagulation factors Biological transport Alkaline earth metals Cellular signal transduction Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Lambert, David G, Contributor Rainbow, Richard D, Contributor ISBN 1627030859 9781627030854 9781627030861 1627030867

Other Titles Calcium Signaling Protocols, 3rd ed

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