| Description |
1 online resource (xi, 204 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1843 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1843. 1064-3745
|
| Contents |
Patch-clamp recording of the CRAC channel current in STIM-Orai overexpressing cells / Yiming Zhang, Sisi Zheng, Yubin Zhou, Donald L. Gill, and Youjun Wang -- Fluorescence-based ratiometric measurement of CRAC channel activity in STIM-Orai-overexpressing HEK-293 cells / Shuce Zhang, Lian He, Yubin Zhou, and Youjun Wang -- Recording SOCE activity in neurons by patch-clamp electrophysiology and microfluorometric calcium imaging / Hsiang-en Wu, Geza Gemes, and Quinn H. Hogan -- Mn2+ quenching assay for store-operated calcium entry / Zui Pan, Sangyong Choi, and Yanhong Luo -- Fluorescence-based measurements of store-operated Ca2+ entry in cancer cells using fluo-4 and confocal live-cell imaging / Fujian Lu, Jianwei Sun, Tao Sun, Heping Cheng, and Shengyu Yang -- Fluorescence-based measurements of the CRAC channel activity in cell populations / Pedro C. Redondo, Alejandro Berna-Erro, Natalia Dionisio, and Juan A. Rosado -- Indirect measurement of CRAC channel activity using NFAT nuclear translocation by flow cytometry in jurkat cells / María D. Carretta, María A. Hidalgo, and Rafael A. Burgos -- CRAC channel components quantitative expression (in tissues and cell lines) using qPCR / Charlotte Dubois, Vyacheslav Lehenkyi, and Natalia Prevarskaya -- Western blotting and co-immunoprecipitation of endogenous STIM/ORAI and protein partners / Charlotte Dubois and Natalia Prevarskaya -- Study of endogenous CRAC channels in human mast cells using an adenoviral delivery system to transduce cells with orai-targeting shRNAs or with cDNAs expressing dominant-negative orai channel mutations / Ian Ashmole and Peter Bradding -- Store-operated Ca2+ entry in Drosophila primary neuronal cultures / Sumita Chakraborty and Gaiti Hasan -- Study of the endogenous CRAC channel using shRNA-mediated gene silencing / Xuexin Zhang, Amy M. Spinelli, Wei Zhang, and Mohamed Trebak -- Engineered cross-linking to study the pore architecture of the CRAC channel / Guolin Ma, Lian He, Ji Jing, Peng Tan, Yun Huang, and Yubin Zhou -- Measurement of the CRAC channel fast Ca2+-Dependent Inactivation (FCDI) / Grigori Y. Rychkov -- High-resolution imaging of STIM/Orai subcellular localization using array confocal laser scanning microscopy / Andras T. Deak, Benjamin Gottschalk, Emrah Eroglu, Rene Rost, Markus Waldeck-Weiermair, Wolfgang F. Graier, and Roland Malli -- Single-channel single-molecule detection (SC-SMD) system / Laura G. Ceballos, Alexander Asanov, and Luis Vaca |
| Bibliography |
Includes bibliographical reference and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed September 10, 2018) |
| Subject |
Calcium channels.
|
|
Calcium Release Activated Calcium Channels
|
|
Calcium Channels
|
|
Biochemistry.
|
|
Science -- Life Sciences -- Biochemistry.
|
|
Calcium channels
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Penna, Aubin, 1977- editor.
|
|
Constantin, Bruno, editor
|
| ISBN |
9781493987047 |
|
1493987046 |
|
