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Title Calcium signaling protocols / edited by David G. Lambert
Edition Second edition
Published Totowa, N.J. : Humana Press, [2006]
©2006
Online access available from:
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Description 1 online resource (x, 359 pages) : illustrations
Series Methods in molecular biology ; v. 312
Methods in molecular biology (Clifton, N.J.) ; volume 312
Contents Fluorescent measurement of [Ca2+]c: basic practical considerations / Alec W.M. Simpson -- Measurement of [Ca2+]i in whole cell suspensions using fura-2 / Robert A. Hirst [and others] -- Measurement of [Ca2+]i in cell suspensions using indo-1 / Adriaan Nelemans -- Confocal microscopy: theory and applications for cellular signaling / Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars -- Single cell and subcellular measurement of intracellular Ca2+ concentration / Anthony J. Morgan and Andrew P. Thomas -- Ratiometric Ca2+ measurements using the FlexStation scanning fluorometer / Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty -- Measuring Ca2+ changes in multiwell format using the fluorometric imaging plate reader / Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty
Measurement of Ca2+ entry into cells using 45Ca2+ / Mercedes Villarroya [and others] -- Measurement of [3H]PN200-110 and [125I] -- conotoxin MVIIA binding / Kazuyoshi Hirota and David G. Lambert -- Whole-cell patch clamp recording of voltage-sensitive Ca2+ channel currents: heterologous expression systems and dissociated brain neurons / Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani -- Measurement of phospholipase C by monitoring inositol phosphates using [3H]inositol-labeling protocols in permeabilized cells / Alison Skippen, Philip Swigart, and Shamshad Cockcroft -- Measurement of inositol(1,4,5)triphosphate using a stereospecific radioreceptor mass assay / Darren Smart
Measurement of calcium fluxes in permeabilized cells using a 45Ca2+ uptake and release assay / Robert A. Wilcox -- Microinjection of myo-inositol(1,4,5)trisphosphate and other calcium-mobilizing agents into intact adherent cells / Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann -- Measurement of free [Ca2+] changes in agonist-sensitive internal stores using compartmentalized fluorescent indicators / Aldebaran M. Hofer -- Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry / Hideo Kanaide -- Measurement of calcium and movement in heart cells / Leong L. Ng and Paulene A. Quinn
Simultaneous analysis of intracellular pH and Ca2+ from cell populations / Raul Martinez-Zaguilan [and others] -- Measurement of cytosolic-free Ca2+ in plant tissue / Martin R. McAinsh and Carl K.-Y. Ng -- Assay and purification of calmodulin-dependent protein kinase / Rajendra K. Sharma -- Assay and purification of calmodulin-dependent cyclic nucleotide phosphodiesterase and isozyme separation / Rajendra K. Sharma -- Measurement of Ca2+-ATPase activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Calcium channels -- Research -- Methodology.
Calcium -- Laboratory manuals.
Calcium -- Research -- Methodology.
Cellular signal transduction -- Research -- Methodology.
Calcium Channels -- pharmacokinetics.
Calcium Signaling -- physiology.
Calcium -- pharmacokinetics.
Signal Transduction.
Spectrometry, Fluorescence -- methods.
Genre/Form Laboratory manuals.
Form Electronic book
Author Lambert, David G.
LC no. 2005005383
ISBN 1588294420 (alk. paper)
1592599494 (e-ISBN)
9781588294425 (alk. paper)
9781592599493 (e-ISBN)