| Description |
1 online resource (x, 359 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 312 |
|
Methods in molecular biology (Clifton, N.J.) ; volume 312
|
| Contents |
Fluorescent measurement of [Ca2+]c: basic practical considerations / Alec W.M. Simpson -- Measurement of [Ca2+]i in whole cell suspensions using fura-2 / Robert A. Hirst [and others] -- Measurement of [Ca2+]i in cell suspensions using indo-1 / Adriaan Nelemans -- Confocal microscopy: theory and applications for cellular signaling / Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars -- Single cell and subcellular measurement of intracellular Ca2+ concentration / Anthony J. Morgan and Andrew P. Thomas -- Ratiometric Ca2+ measurements using the FlexStation scanning fluorometer / Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty -- Measuring Ca2+ changes in multiwell format using the fluorometric imaging plate reader / Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty |
|
Measurement of Ca2+ entry into cells using 45Ca2+ / Mercedes Villarroya [and others] -- Measurement of [3H]PN200-110 and [125I] -- conotoxin MVIIA binding / Kazuyoshi Hirota and David G. Lambert -- Whole-cell patch clamp recording of voltage-sensitive Ca2+ channel currents: heterologous expression systems and dissociated brain neurons / Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani -- Measurement of phospholipase C by monitoring inositol phosphates using [3H]inositol-labeling protocols in permeabilized cells / Alison Skippen, Philip Swigart, and Shamshad Cockcroft -- Measurement of inositol(1,4,5)triphosphate using a stereospecific radioreceptor mass assay / Darren Smart |
|
Measurement of calcium fluxes in permeabilized cells using a 45Ca2+ uptake and release assay / Robert A. Wilcox -- Microinjection of myo-inositol(1,4,5)trisphosphate and other calcium-mobilizing agents into intact adherent cells / Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann -- Measurement of free [Ca2+] changes in agonist-sensitive internal stores using compartmentalized fluorescent indicators / Aldebaran M. Hofer -- Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry / Hideo Kanaide -- Measurement of calcium and movement in heart cells / Leong L. Ng and Paulene A. Quinn |
|
Simultaneous analysis of intracellular pH and Ca2+ from cell populations / Raul Martinez-Zaguilan [and others] -- Measurement of cytosolic-free Ca2+ in plant tissue / Martin R. McAinsh and Carl K.-Y. Ng -- Assay and purification of calmodulin-dependent protein kinase / Rajendra K. Sharma -- Assay and purification of calmodulin-dependent cyclic nucleotide phosphodiesterase and isozyme separation / Rajendra K. Sharma -- Measurement of Ca2+-ATPase activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka |
| Summary |
Now extensively updated and expanded, Calcium Signaling Protocols, Second Edition continues the excellence of the highly regarded first edition. New to this edition are chapters on the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluorometric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many facets of Ca2+ signaling. The protocols follow the successful Methods in Molecular Biology"!series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Calcium Signaling Protocols, Second Edition provides for novice and expert researchers alike both theoretical and practical information for the optimal design and performance of studies of intracellular Ca2+ regulation |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Calcium -- Laboratory manuals
|
|
Calcium -- Research -- Methodology
|
|
Calcium channels -- Research -- Methodology
|
|
Cellular signal transduction -- Research -- Methodology
|
|
Cellular signal transduction.
|
|
Calcium -- pharmacokinetics
|
|
Calcium Channels -- pharmacokinetics
|
|
Calcium Signaling -- physiology
|
|
Signal Transduction
|
|
Spectrometry, Fluorescence -- methods
|
|
SCIENCE -- Life Sciences -- Biochemistry.
|
|
Cellular signal transduction
|
|
Calcium
|
|
Cellular signal transduction -- Research -- Methodology
|
|
Calcium.
|
|
Celbiologie.
|
|
Wetenschappelijke technieken.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Lambert, David G
|
| ISBN |
1588294420 |
|
9781588294425 |
|
9781592599493 |
|
1592599494 |
|
