| Description |
1 online resource (xxi, 817 pages) : illustrations |
| Series |
Methods in molecular medicine ; 39 |
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Methods in molecular medicine ; 39.
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| Contents |
The epidemiology of ovarian cancer / E. Banks-Familial ovarian cancer / R.P. Zweemer and I.J. Jacobs-The molecular pathogenesis of ovarian cancer / S.E. Hilary Russell-Alterations in oncogenes, tumor suppressor genes, and growth factors associated with epithelial ovarian cancers / R.C. Bast, Jr. and G.B. Mills-Pathological assessment of ovarian cancer / A.R. Williams-Tumor markers in screening for ovarian cancer / S.J. Skates, I.J. Jacobs and R.C. Knapp-Primary surgical management of ovarian cancer / D.S. Chi and W.J. Hoskins-Recent insights into drug resistance in ovarian cancer / T.C. Hamilton and S.W. Johnson-Markers of tumor burden : An overview / J.E. Roulston-Bioactive interleukin-6 levels in serum and ascites as a prognostic factor in patients with epithelial ovarian cancer / G. Gastl and M. Plante-Elisa-based quantification of p105 (c-erbb-2, her2/neu) in serum of ovarian carcinoma / H. Meden, A. Fattahi-Meibodi and D. Marx-Enzyme immunoassay of urinary beta-core fragment of human chorionic gonadotropin as a tumor marker for ovarian cancer / R. Nishimura [and others]-Ovarian cancer models : Technical review / S.P. Langdon, J. Edwards and J.M. Bartlett-Establishment of ovarian cancer cell lines / S.P. Langdon and S.S. Lawrie-Subcloning of ovarian cancer cell lines / T.W. Grunt-Culture and characterization of human ovarian surface epithelium / N. Auersperg and S.L. Maines-Bandiera-Mtt growth assays in ovarian cancer / D.M. Spinner-In vitro invasion assays / S.K. Chambers-P53transfectants in ovarian cancer / F. Vikhanskaya and M. Broggini-Estrogen-responsive ovarian cancer xenografts / A.A. Ritchie and S.P. Langdon-The use of matrigel in the establishment of ovarian carcinoma cell lines as xenografts / P. Mullen and S.P. Langdon-Cytogenetics of ovarian cancer technical overview / T. Liehr-Interphase cytogenetics in frozen ovarian tumor tissue / T. Liehr-Interphase cytogenetics in paraffin-embedded ovarian tissue / S. Neubauer and T. Liehr-Rapid identification of chromosomes using primed in situ labeling (prins) / G.V. Velagaleti-Gene amplification of c-erbb-2 detected by fish / S.R. Young, W.H. Liu and Z.R. Wang-Chromosome microdissection for detection of subchromosomal alterations by fish / X.Y. Guan and J.M. Trent-Quantitation of fish signals in archival tumors / A.D. Watters and J.M. Bartlett-Comparative genomic hybridization for the analysis of unbalanced chromosomal abnormalities in ovarian tumors / L.J. Curtis-Molecular genetics of ovarian cancer : A technical overview / W. Foulkes and A.N. Shelling-Extraction of DNA from microdissected archival tissues / J.J. Going-Rflp molecular analysis of the urokinase-type plasminogen activator gene / B. Muehlenweg [and others]-Pcr microsatellite analysis of loh in ovarian tumors / J. Devlin and M.A. Knowles-Molecular genetic analysis of flow-sorted and microdissected ovarian tumor cells improved detection of loss of heterozygosity / E.C. Abeln and W.E. Corver-Sscp and sequence analysis of p53 mutations in ovarian tumors / A.K. Sood and R.E. Buller-Multiplex pcr (mpcr) screening can detect small intragenic p53 deletion and insertion mutations / I.B. Runnebaum and S. Wang-Gohrke-Transfer of human chromosome 3 to an ovarian carcinoma cell line identifies regions involved in ovarian cancer / P. Rimessi and F. Gualandi-Double- and competitive-differential pcr for gene dosage quantitation / B. Brandt [and others]-One-dimensional genome scanning-detection of genomic changes in ovarian carcinoma / A. Magliocco and M. Brilliant-Mapping of tumor suppressor genes in ovarian cancers / I.G. Campbell and E.J. Bryan-Detection of the replication error phenotype in ovarian cancer-pcr analysis of microsatellite instability / G.L. Hirst and R. Brown-Immunostaining human paraffin-embedded sections for mismatch repair proteins / M. Mackean and R. Brown-Quantitative pcr detection of c-erbb-2 gene amplification / K. Dobianer, M. Medl and J. Spona-Mrna analyses : A technical overview / J.M. Bartlett-In situ hybridization detection of tgf-beta mrna / A. Gobl and R. Henriksen-Mrna detection by in situ rt-pcr / H. Anne Waller and A. Kay Savage-Rnase protection assay analysis of mrna for tgfbeta1( -3) in ovarian tumors / J.M. Bartlett-Rt-pcr quantitation of hsp60 mrna expression / R.P. Perez [and others]-The effects of butyrate and the role of c-myc in n.1 ovarian carcinoma cells determined by northern blotting / G. Krupitza-Differential display / G.C. Sellar [and others]-Measurement of protein expression a technical overview / J.R. Reeves and J.M. Bartlett-Measurement of igf-1 receptor content in tissues and cell lines by radioimmunoassay (ria) and elisa techniques / E.P. Beck [and others]-Radioreceptor measurement of er/pr giovanni scambia, gabriella ferrandina, g. D'agostino / A.D. Dilectis and S. Mancuso-Nm23 protein expression by western blotting in patients with epithelial ovarian carcinoma / G. Scambia [and others]-Detection and quantitation of matrix metalloproteases by zymography / T.M. Leber and R.P. Negus-Simultaneous flow cytometric detection of DNA and cellular protein molecules / W.E. Corver and C.J. Cornelisse-C-erbb-2 immunohistochemistry in paraffin tumors / G.H. Eltabbakh-Quantitative immunohistochemistry of estrogen and progesterone receptor positivity in ovarian tumors : A rapid and reliable stereological approach / G.A. Meijer [and others]-Radioimmunohistochemistry : Quantitative analysis of cell surface receptors in frozen sections / J.R. Reeves-Signal transduction : A technical overview / M.J. Arboleda and D.J. Slamon-Immunoprecipitation to determine jak kinase activation in response to interleukins in ovarian cancer / T. Murata-Detection of erbb receptor family tyrosyl phosphorylation in ovarian carcinoma cells / A.W. Hamburger-Phosphotyrosine kinase assays as a prescreen for inhibitors of egfr / J. Edwards and J.M. Bartlett-Direct kinase assay screening for inhibitors of map kinase / J. Edwards and J.M. Bartlett-Phosphatidylinositol kinase activity in ovarian cancer cells / A. Imai [and others]-Induction of c-fos gene expression by urokinase-type plasminogen activator in human ovarian cancer cells / I. Dumler-Phosphotyrosine phosphatase activity in ovarian carcinoma cells : Stimulation by gnrh in plasma membrane / A. Imai [and others]-Phosphatidylinositol 4-kinase assay in ovarian carcinoma cells / S. Isonishi [and others]-Calcium mobilization in ovarian cancer cells in response to lysophospholipids / A.Y. Xu and D.S. Damron-Compartmentalized protein kinase c activation in ovarian carcinoma cells / A. Basu and G.R. Akkaraju-A novel and simple method to assay the activity of individual protein kinases in a crude tissue extract / B.S. Goueli, K. Hsiao and S.A. Goueli-Photoaffinity detection of camp binding proteins in ovarian cancer / D.J. Burns and M.J. Hulme-Apoptosis : A technical overview / S.M. Quirk-Detection of low levels of DNA fragmentation in ovarian tumor cell lines / A.J. McIlwrath-Measurement of apoptotic cells by flow cytometry (tunnel assay) / M.G. Ormerod-Detection of apoptosis in ovarian cells in vitro and in vivo using the annexin v-affinity assay / M. Engeland [and others]-Use of diphenylamine in the detection of apoptosis / R.K. Gibb and C. Gercel-Taylor-Activation of caspase protease during apoptosis in ovarian cancer cells / Z. Chen [and others]-Differential expression of apoptosis-associated genes bax and bcl-2 in ovarian cancer / D. Marx and H. Meden-DNA-dependent protein kinase in apoptosis / J.J. Turchi-Detection of telomerase activity by pcr-based assay / S. Kyo-Immunological and gene therapy for ovarian cancer a technical overview / C. Gourley and H. Gabra-Developing anti-her2/neu single-chain fv fragments from phage display libraries / G.P. Adams and R. Schier-Direction of the recognition specificity of cytotoxic t cells toward tumor cells by transduced, chimeric t-cell receptor genes / M. Maurer-Gebhard [and others]-Eradication of c-erbb-2-positive ovarian cancer cells mediated by intracellular expression of anti-c-erbb-2 antibody / J. Deshane, R.D. Alvarez and G.P. Siegal-C-erbb-2 antisense oligonucleotides inhibit serum-induced cell spreading of ovarian cancer cells / K. Wiechen-E1a-mediated gene therapy / M.C. Hung, D.H. Yan and S. Zhang-P53 adenovirus as gene therapy for ovarian cancer / J.L. Carroll [and others]-Bispecific antibody mdx-210 for treatment of advanced ovarian and breast cancer / P.A. Kaufman [and others] |
| Summary |
Cancers are highly complex disease systems that require access to widely diverse laboratory techniques from a broad range of research disciplines, methods that are often beyond the knowledge of a single researcher. In Ovarian Cancer: Methods and Protocols, over one hundred well-respected scientists comprehensively review every aspect of the biology underlying ovarian cancer and present a detailed collection of easy-to-follow methods for illuminating this frequently fatal disease's basic mechanisms. Here today's investigators will find the broadest imaginable array of molecular biology techniques specially designed to determine not only tumor genetics, expression, and protein function, but also to elucidate the genetic mechanisms by which gene and immunotherapies may be perfected. The book's highly experienced authors provide help in selecting the correct model system for each experimental approach, and offer extensive technical advice to facilitate the planning of individual experiments and ensure that researchers are using the optimal techniques for their research programs. Although structured primarily for ovarian cancer researchers, and using examples from that field, the techniques can be immediately applied to research in many other tumor types. Versatile and comprehensive, Ovarian Cancer Methods and Protocols constitutes a veritable treasury of well-proven expert methods that will help basic scientists and clinical researchers alike master the powerful technologies required for effective cancer research today |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Ovaries -- Cancer -- Molecular aspects -- Research -- Methodology
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Tumor markers -- Research -- Methodology
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Ovaries -- Cancer -- Genetic aspects -- Research -- Methodology
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Biological models.
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Tumor markers.
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Ovarian Neoplasms -- genetics
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Models, Biological
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Molecular Biology
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Biomarkers, Tumor
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MEDICAL -- Oncology.
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HEALTH & FITNESS -- Diseases -- Cancer.
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Tumor markers
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Biological models
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Ovaries -- Cancer -- Genetic aspects -- Research -- Methodology
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Ovaries -- Cancer -- Molecular aspects -- Research -- Methodology
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Kanker.
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Ovaria.
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Tumoren.
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Wetenschappelijke technieken.
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| Genre/Form |
Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Bartlett, John M. S.
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| ISBN |
9781592590711 |
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1592590713 |
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