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Title Subcellular fractionation : a practical approach / edited by J.M. Graham and D. Rickwood
Published Oxford ; New York : IRL Press at Oxford University Press, [1997]


Location Call no. Vol. Availability
 MELB  571.6078 Gra/Sfa  AVAILABLE
Description xx, 339 pages : illustrations ; 24 cm
Series The practical approach series ; 173
Practical approach series ; 173
Contents 1. Homogenization of tissues and cells / John M. Graham -- 2. Isolation of subcellular fractions / Richard H. Hinton and Barbara M. Mullock -- 3. Isolation and characterization of nuclei and nuclear subfractions / David Rickwood, Anthea Messent and Dipak Patel -- 4. Subcellular fractionation of mitochondria / J. E. Rice and J. G. Lindsay -- 5. Isolation and characterization of peroxisomes / A. Volkl, E. Baumgart and H. D. Fahimi -- 6. Lysosomes and endocytosis / Tor Gjoen, Trond Olav Berg and Trond Berg -- 7. The membranes of the secretory and exocytic pathways / John M. Graham -- 8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells / Anthony L. Moore and David G. Whitehouse -- 9. Ribosomes and polysomes / Ulrich A. Bommer, Nils Burkhardt and Ralf Junemann [et al.] -- 10. Electron microscopy of organelles / Nigel James -- A1. Core list of suppliers
Summary Many investigations into cell structure and function require the isolation of a particular subcellular particle. Subcellular Fractionation covers the subject comprehensively, describing in detail the wide range of separation techniques and characterization procedures for all the major subcellular organelles: nuclei, mitochondria, chloroplasts, peroxisomes, and the membrane systems of the exocytic and endocytic pathways. Importantly, the text also describes the isolation of chromosomes, nucleoli and nucleoprotein complexes, and key procedures related to the analysis of these particles, such as the labelling of ligands, kinetic analysis of their internalization, and electron microscopy
Analysis fractions nuclear subfractions nuclei mitochondria peroxisomes lysoso mes endocytosis secretory pathways ribosomes polysomes organelles
Notes Includes index
Bibliography Includes bibliographical references and index
Subject Cell fractionation -- Handbooks, manuals, etc.
Cell fractionation -- Laboratory manuals.
Subcellular fractionation -- Handbooks, manuals, etc.
Subcellular fractionation -- Laboratory manuals.
Subcellular Fractions -- analysis
Subcellular Fractions -- methods
Genre/Form Handbooks and manuals.
Author Graham, J. M. (John M.), 1943-
Rickwood, D. (David)
LC no. 97179028
ISBN 0199634947 (paperback)