| Description |
1 online resource (xx, 404 pages, 4 unnumbered pages of plates) : illustrations (some color) |
| Contents |
Organization and good aseptic technique in the human embryonic stem cell laboratory / Minal J. Patel, Emma L. Stephenson and Stephen L. Minger -- Sourcing human embryonic stem cell lines / Glyn Stacey -- Culture of human embryos for stem cell derivation / R. Douglas Powers and Jeannine Witmyer -- Derivation of human embryonic stem cell lines / Stephen Sullivan [and others] -- Standard culture of human embryonic stem cells / Jeremy M. Crook, Rachel Horne and Alan Colman -- Chemically-defined culture of human embryonic stem cells / Julie Hsu Clark and Sheng Ding -- Phenotypic analysis of human embryonic stem cells / Jonathan S. Draper, Cheryle A. Séguin and Peter W. Andrews -- Genetic and epigenetic analysis of human embryonic stem cells / Laurie A. Boyer, Rudolf Jaenisch and Maisam Mitalipova -- In vivo characterization of human embryonic stem cells / Scott A. Noggle, Francesca M. Spagnoli and Ali H. Brivanlou -- In vitro differentiation of human embryonic stem cells / A. Henry Sathananthan and Alan Trounson -- Differentiation of human embryonic stem cells into extraembryonic cell types / Cheryle A. Séguin and Jonathan S. Draper -- Directed differentiation of human embryonic stem cells into endoderm / Kenji Osafune [and others] -- Directed differentiation of human embryonic stem cells into mesoderm / Christine Mummery [and others] -- Directed differentiation of human embryonic stem cells into ectoderm / Emily A. Davis [and others] -- Gene targeting in human embryonic stem cells : knock out and knock in by homologous recombination / Thomas P. Zwaka -- RNA interference in human embryonic stem cells / M. William Lensch, Asmin Tulpule and Holm Zaehres -- Generation of human gene reporters using bacterial artificial chromosome recombineering / Andrew J. Washkowitz and David A. Shaywitz |
| Summary |
With this valuable practical guide, three members of the Harvard Stem Cell Institute have compiled and edited the definite handbook for the exciting new field of human embryonic stem cell research. The editors have gathered protocols from scientists with extensive reputation and expertise, describing and comparing currently used techniques for the culture of human stem cells and discussing the strengths and weaknesses of the different approaches |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Embryonic stem cells -- Research.
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Fetus -- Research.
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Cell Culture Techniques -- methods.
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Cell Differentiation -- physiology.
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Cell Line -- physiology.
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Embryonic Stem Cells -- physiology.
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| Form |
Electronic book
|
| Author |
Cowan, Chad A.
|
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Eggan, Kevin.
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Sullivan, Stephen, Dr.
|
| ISBN |
0470511591 (electronic bk.) |
|
0470511613 |
|
1280900903 |
|
9780470511596 (electronic bk.) |
|
9780470511619 |
|
9781280900907 |
|
