| Description |
1 online resource (xiv, 405 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; 1749 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1749. 1940-6029
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| Contents |
Random migration assays of mammalian cells and quantitative analyses of single cell trajectories / Irene Dang and Alexis Gautreau -- Directional collective migration in wound healing assays / Nicolas Molinie and Alexis Gautreau -- In vitro system to study the mesenchymal-to-amoeboid transition / Aleksandra S. Chikina and Antonina Y. Alexandrova -- In vitro system to study the epithelial-mesenchymal transition in vitro / Natalya A. Gloushankova, Svetlana N. Rubtsova, and Irina Y. Zhitnyak -- Detection of migrasomes / Yang Chen, Ying Li, Liang Ma, and Li Yu -- 3D endothelial cell migration / Kathryn A. Jacobs and Julie Gavard -- Transmigration of leukocytes across epithelial monolayers / Penny E. Morton and Maddy Parsons -- Evaluation of tumor cell invasiveness in vivo : the chick chorioallantoic membrane assay / Selma Maacha and Simon Saule -- Analysis of invasion dynamics of matrix-embedded cells in a multisample format / Marleen Van Troys, Paola Masuzzo, Lynn Huyck, Karima Bakkali, Davy Waterschoot, Lennart Martens, and Christophe Ampe -- Using systems microscopy to understand the emergence of cell migration from cell organization / Staffan Strömblad and John G. Lock -- Neuronal precursor migration in ex vivo brain slice culture / Fu-Sheng Chou and Pei-Shan Wang -- In vitro models to analyze the migration of MGE-derived interneurons / Claire Leclech and Christine Métin -- Cell migration in tissues : explant culture and live imaging / Ralitza Staneva, Jorge Barbazan, Anthony Simon, Danijela Matic Vignjevic, and Denis Krndija -- Intravital imaging of tumor cell motility in the tumor microenvironment context / Battuya Bayarmagnai, Louisiane Perrin, Kamyar Esmaeili Pourfarhangi, and Bojana Gligorijevic -- Using the zebrafish embryo to dissect the early steps of the metastasis cascade / Gautier Follain, Naël Osmani, Cédric Fuchs, Guillaume Allio, Sébastien Harlepp, and Jacky G. Goetz -- Analysis of in vivo cell migration in mosaic zebrafish embryos / Arthur Boutillon, Florence A. Giger, and Nicolas B. David -- Analysis of cell shape and cell migration of drosophila macrophages in vivo / Marike Rüder, Benedikt M. Nagel, and Sven Bogdan -- Migration of Q cells in Caenorhabditis elegans / Yongping Chai, Zhiwen Zhu, and Guangshuo Ou -- Imaging the molecular machines that power cell migration / Anika Steffen, Frieda Kage, and Klemens Rottner -- Biologist-friendly method to analyze cross-correlation between protrusion dynamics and membrane recruitment of actin regulators / Perrine Paul-Gilloteaux, François Waharte, Manish Kumar Singh, and Maria Carla Parrini -- Using single-protein tracking to study cell migration / Thomas Orré, Amine Mehidi, Sophie Massou, Olivier Rossier, and Grégory Giannone -- Optogenetic control of cell migration / Xenia Meshik, Patrick R. O'Neill, and N. Gautam -- Electrotaxis : cell directional movement in electric fields / Jolanta Sroka, Eliza Zimolag, Slawomir Lasota, Wlodzimierz Korohoda, and Zbigniew Madeja -- Analysis of random migration of Dictyostelium amoeba in confined and unconfined environments / Christof Litschko, Julia Damiano-Guercio, Stefan Brühmann, and Jan Faix -- Neutrophil chemotaxis in one droplet of blood using microfluidic assays / Xiao Wang and Daniel Irimia -- Leukocyte migration and deformation in collagen gels and microfabricated constrictions / Pablo J. Sáez, Lucie Barbier, Rafaele Attia, Hawa-Racine Thiam, Matthieu Piel, and Pablo Vargas -- Microfluidic devices for examining the physical limits of migration in confined environments / Majid Malboubi, Asier Jayo, Maddy Parsons, and Guillaume Charras -- Controlling confinement and topology to study collective cell behaviors / Guillaume Duclos, Maxime Deforet, Hannah G. Yevick, Olivier Cochet-Escartin, Flora Ascione, Sarah Moitrier, Trinish Sarkar, Victor Yashunsky, Isabelle Bonnet, Axel Buguin, and Pascal Silberzan |
| Summary |
This volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. Some of the topics explored in this book are: migration in confined environments, microfluidic devices, optogenetics, chemotaxis, electrotaxis, detection of migrasomes, migration of Q cells in Caenorhabditis elegans, of Drosophila macrophages, optogenetics of cell migration, intravital imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Cell Migration: Methods and Protocols is a valuable resource for anyone interested in learning more about this expanding field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerProtocol, viewed March 12, 2018) |
| Subject |
Cell migration -- Laboratory manuals
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Cell Movement
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Cellular biology (cytology)
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Science -- Life Sciences -- Biology -- Molecular Biology.
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Cell migration
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Gautreau, Alexis, editor.
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| ISBN |
9781493977017 |
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1493977016 |
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