| Description |
1 online resource (xv, 364 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; 294 |
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Methods in molecular biology (Clifton, N.J.) ; v. 294.
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| Contents |
1 Cell Migration: An Overview / Donna J. Webb, Huaye Zhang, Alan F. Horwitz 3 -- Part II Basic Cell Migration and Related Assays -- 2 Boyden Chamber Assay / Hong-Chen Chen 15 -- 3 Wound-Healing Assay / Luis G. Rodriguez, Xiaoyang Wu, Jun-Lin Guan 23 -- 4 Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy / Claire M. Wells, Anne J. Ridley 31 -- 5 Cell-Adhesion Assays / Dennis F. Kucik, Chuanyue Wu 43 -- 6 Cell-Spreading Assays / Allison L. Berrier, Susan E. LaFlamme 55 -- 7 Cell-Scatter Assay / Hong-Chen Chen 69 -- 8 Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices / Edna Cukierman 79 -- Part III Analysis of Cell Migration in Specialized Cell Systems -- 9 Tumor Cell Invasion Assays / Leslie M. Shaw 97 -- 10 Analysis of Endothelial Cell Migration Under Flow / Song Li 107 -- 11 Angiogenesis Assays in the Chick CAM / Chris Storgard, David Mikolon, Dwayne G. Stupack 123 -- 12 Investigations of Neuronal Migration in the Central Nervous System / Michael E. Ward, Yi Rao 137 -- Part IV Analysis of Cell Migration in Model Organisms -- 13 Analysis of Cell Migration in Caenorhabditis elegans / M. Afaq Shakir, Erik A. Lundquist 159 -- 14 Analysis of Cell Migration Using Drosophila as a Model System / Jocelyn A. McDonald, Denise J. Montell 175 -- 15 Imaging Cell Movement During Dorsal Closure in Drosophila Embryos / William Wood, Antonio Jacinto 203 -- 16 Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell Populations In Vivo / Diane S. Sepich, Lilianna Solnica-Krezel 211 -- 17 The Xenopus Embryo as a Model System for Studies of Cell Migration / Douglas W. DeSimone, Lance Davidson, Mungo Marsden, Dominique Alfandari 235 -- 18 Neural Crest Migration Methods in the Chicken Embryo / Maria Elena de Bellard, Marianne Bronner-Fraser 247 -- 19 In Vivo and In Vitro Models of Mammalian Angiogenesis / Mien V. Hoang, Donald R. Senger 269 -- Part V Biochemical and Novel Approaches in Cell Migration -- 20 Measurement of Protein Tyrosine Phosphorylation in Cell Adhesion / Vidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, Michael D. Schaller 289 -- 21 Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Membrane Patches / Frauke Drees, Amy Reilein, W. James Nelson 303 -- 22 Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells / Christoph Ballestrem, Benjamin Geiger 321 -- 23 Use of Multiphoton Imaging for Studying Cell Migration in the Mouse / Andrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, Alexander Yu. Nikitin 335 -- 24 Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration / Daniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, Jun-Lin Guan 347 |
| Summary |
Central to the better understanding of both molecular mechanisms and disease, cell migration plays an essential role in a variety of biological processes and is now the subject of intense study using an array of powerful new technologies. In Cell Migration: Developmental Methods and Protocols, researchers describe in step-by-step detail their most successful techniques for studying the macromolecular machinery of cell movement. These readily reproducible protocols include a wide range of novel and state-of-the-art methodologies, as well as many classic methods, for use in cultured cells, different model organisms, and specialized cells in both normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The authors also offer several novel approaches to the study of cell migration, as well as extensive coverage of cell migration studies in developmental and disease models. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and highly practical, Cell Migration: Developmental Methods and Protocols offers researchers easy access to many readily reproducible techniques for the optimally productive investigation of cell migration in today's interdisciplinary experimental environment |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
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Print version record |
| Subject |
Cell migration -- Laboratory manuals
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Cell Movement -- physiology
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Biological Assay -- methods
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Cell Movement -- genetics
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SCIENCE -- Life Sciences -- Cell Biology.
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Cell migration
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Guan, Jun-Lin
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| ISBN |
9781588293824 |
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1588293823 |
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9781617375323 |
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1617375322 |
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9781592598601 |
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1592598609 |
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